Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/recombinant-production-crystallization-and-preliminary-x-ray-analysis-lW05y4k4H6
References (25)
-
George-Lucian Moldovan, B. Pfander, S. Jentsch (2007)
PCNA, the Maestro of the Replication ForkCell, 129
-
R. Cavicchioli, K. Siddiqui, D. Andrews, K. Sowers (2002)
Low-temperature extremophiles and their applications.Current opinion in biotechnology, 13 3
-
B. Matthews (1968)
Solvent content of protein crystals.Journal of molecular biology, 33 2
-
X. Kong, R. Onrust, M. O’Donnell, J. Kuriyan (1992)
Three-dimensional structure of the β subunit of E. coli DNA polymerase III holoenzyme: A sliding DNA clampCell, 69
-
I. Dionne, R. Nookala, S. Jackson, A. Doherty, S. Bell (2003)
A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.Molecular cell, 11 1
-
E. Warbrick (2000)
The puzzle of PCNA's many partnersBioEssays, 22
-
W. Cardoso, D. Kotton (2008)
Figure 3 -
Andrew Doré, M. Kilkenny, Sarah Jones, Antony Oliver, S. Roe, Stephen Bell, Laurence Pearl (2006)
Structure of an archaeal PCNA1–PCNA2–FEN1 complex: elucidating PCNA subunit and client enzyme specificityNucleic Acids Research, 34
-
Z. Otwinowski, W. Minor (1997)
[20] Processing of X-ray diffraction data collected in oscillation mode.Methods in enzymology, 276
-
S. Naryzhny, L. Desouza, K. Siu, Hoyun Lee (2006)
Characterization of the human proliferating cell nuclear antigen physico-chemical properties: aspects of double trimer stability.Biochemistry and cell biology = Biochimie et biologie cellulaire, 84 5
-
T. Krishna, X. Kong, S. Gary, P. Burgers, J. Kuriyan (1994)
Crystal structure of the eukaryotic DNA polymerase processivity factor PCNACell, 79
-
L. García‐ortega, V. Ríos, A. Martínez-Ruíz, M. Oñaderra, J. Lacadena, A. Pozo, J. Gavilanes (2005)
Anomalous electrophoretic behavior of a very acidic protein: Ribonuclease U2ELECTROPHORESIS, 26
-
S. Naryzhny, Hoyun Lee (2004)
The Post-translational Modifications of Proliferating Cell Nuclear AntigenJournal of Biological Chemistry, 279
-
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
-
S. Matsumiya, Y. Ishino, K. Morikawa (2001)
Crystal structure of an archaeal DNA sliding clamp: Proliferating cell nuclear antigen from Pyrococcus furiosusProtein Science, 10
-
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
-
E. Warbrick (1998)
PCNA binding through a conserved motif.BioEssays : news and reviews in molecular, cellular and developmental biology, 20 3
-
G. Maga, U. Hübscher (2003)
Proliferating cell nuclear antigen (PCNA): a dancer with many partnersJournal of Cell Science, 116
-
大房 健 (2005)
基礎講座 電気泳動(Electrophoresis) -
C. Gerday, M. Aittaleb, Mostafa Bentahir, J. Chessa, P. Claverie, Tony Collins, S. D'amico, Joëlle Dumont, G. Garsoux, D. Georlette, A. Hoyoux, Thierry Lonhienne, M. Meuwis, G. Feller (2000)
Cold-adapted enzymes: from fundamentals to biotechnology.Trends in biotechnology, 18 3
-
P. Franzmann, N. Springer, W. Ludwig, E. Macario, M. Rohde (1992)
A Methanogenic Archaeon from Ace Lake, Antarctica: Methanococcoides burtonii sp. nov.Systematic and Applied Microbiology, 15
-
B. Chapados, D. Hosfield, Seungil Han, J. Qiu, B. Yelent, B. Shen, J. Tainer (2004)
Structural Basis for FEN-1 Substrate Specificity and PCNA-Mediated Activation in DNA Replication and RepairCell, 116
-
K. Mullis, F. Faloona (1987)
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.Methods in enzymology, 155
-
(2004)
Cell , 116 , 39 – 50 . Collaborative Computational Project , Number 4 ( 1994 ) -
N. Stein (2008)
CHAINSAW: a program for mutating pdb files used as templates in molecular replacementJournal of Applied Crystallography, 41
- Publisher
- Wiley
- Copyright
- International Union of Crystallography, 2009
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309109037075
- pmid
- 19923734
- Publisher site
- See Article on Publisher Site
Abstract
Proliferating cell nuclear antigen (PCNA) is a DNA‐clamping protein that is responsible for increasing the processivity of the replicative polymerases during DNA replication and repair. The PCNA from the eurypsychrophilic archaeon Methanococcoides burtonii DSM 6242 (MbPCNA) has been targeted for protein structural studies. A recombinant expression system has been created that overproduces MbPCNA with an N‐terminal hexahistidine affinity tag in Escherichia coli. As a result, recombinant MbPCNA with a molecular mass of 28.3 kDa has been purified to at least 95% homogeneity and crystallized by vapor‐diffusion equilibration. Preliminary X‐ray analysis revealed a trigonal hexagonal R3 space group, with unit‐cell parameters a = b = 102.5, c = 97.5 Å. A single MbPCNA crystal was subjected to complete diffraction data‐set collection using synchrotron radiation and reflections were measured to 2.40 Å resolution. The diffraction data were of suitable quality for indexing and scaling and an unrefined molecular‐replacement solution has been obtained.
Journal
Acta Crystallographica Section F – Wiley
Published: Nov 1, 2009