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M. Sandrin, H. Vaughan, I. Mckenzie (1994)
Identification of Gal(α1,3)Gal as the major epitope for pig-to-human vascularised xenograftsTransplantation Reviews, 8
Shikhman (1994)
A sub set of mouse monoclonal antibodies cross reactive with cytoskeletal proteins and group A streptococcal M proteins recognise N-acetyl-β-D-glucosamineJ Immunol, 153
R. Merrifield (1965)
Automated synthesis of peptides.Science, 150 3693
M. Sandrin, I. Mckenzie (1994)
Galα(1,3)Gal, the Major Xenoantigen(s) Recognised in Pigs by Human Natural AntibodiesImmunological Reviews, 141
A. Varki (1993)
Biological roles of oligosaccharides: all of the theories are correctGlycobiology, 3
C. Wood, C. Wood, E. Kabat, E. Kabat, L. Murphy, L. Murphy, I. Goldstein, I. Goldstein (1979)
Immunochemical studies of the combining sites of the two isolectins, A4 and B4, isolated from Bandeiraea simplicifolia.Archives of biochemistry and biophysics, 198 1
A. Shikhman, M. Cunningham (1994)
Immunological mimicry between N-acetyl-beta-D-glucosamine and cytokeratin peptides. Evidence for a microbially driven anti-keratin antibody response.Journal of immunology, 152 9
Jamie Scott, Duraikkannu LOGANATHANt, R. Easley, Xiaofen Gong, Irwin GOLDSTEINt (1992)
A family of concanavalin A-binding peptides from a hexapeptide epitope library.Proceedings of the National Academy of Sciences of the United States of America, 89
C. Hayes, I. Goldstein (1974)
An alpha-D-galactosyl-binding lectin from Bandeiraea simplicifolia seeds. Isolation by affinity chromatography and characterization.The Journal of biological chemistry, 249 6
Hayes (1974)
An α-D-galactosyl-binding lectin from Bandeira simplicifolia seeds. Isolation by affin ity chromatographyJ Biol Chem, 249
Vaughan (1991)
The isolation of cDNA clones for CD48Immunogenetics, 33
H. Vaughan, I. Mckenzie, M. Sandrin (1995)
Biochemical studies of pig xenoantigens detected by naturally occurring human antibodies and the galactose alpha(1-3)galactose reactive lectin.Transplantation, 59 1
Ian McKenzie, P. Xing, H. Vaughan, J. Prenzoska, P. Dabkowski, M. Sandrin (1994)
Distribution of the major xenoantigen (gal (alpha 1-3)gal) for pig to human xenografts.Transplant immunology, 2 2
A. Shikhman, Neil Greenspan, Madeleine Cunningham (1994)
Cytokeratin peptide SFGSGFGGGY mimics N-acetyl-beta-D-glucosamine in reaction with antibodies and lectins, and induces in vivo anti-carbohydrate antibody response.Journal of immunology, 153 12
R. Hoess, U. Brinkmann, T. Handel, I. Pastan (1993)
Identification of a peptide which binds to the carbohydrate-specific monoclonal antibody B3.Gene, 128 1
M. Sandrin, H. Vaughan, P. Dabkowski, I. McKenzie (1993)
Anti-pig IgM antibodies in human serum react predominantly with Gal(alpha 1-3)Gal epitopes.Proceedings of the National Academy of Sciences of the United States of America, 90 23
Kevin Oldenburg, Duraikkannu Loganathan, Irwin Goldstein, Peter Schultz, M. Gallop (1992)
Peptide ligands for a sugar-binding protein isolated from a random peptide library.Proceedings of the National Academy of Sciences of the United States of America, 89
H. Vaughan, B. Loveland, M. Sandrin (1994)
Gal alpha(1,3)Gal is the major xenoepitope expressed on pig endothelial cells recognized by naturally occurring cytotoxic human antibodies.Transplantation, 58 8
U. Galili, R. Mandrell, Randa Hamadeh, S. Shohet, J. Griffiss (1988)
Interaction between human natural anti-alpha-galactosyl immunoglobulin G and bacteria of the human floraInfection and Immunity, 56
Abstract: Strategies are now being designed to overcome the hyperacute rejection of vascularized pig xenografts by human natural anti‐Galα(1,3)Gal antibodies. We now demonstrate that a synthetic octapeptide, Gal pep 1 (DAHWESWL), isolated from a peptide epitope library using the α‐galactosyl specific lectin IB4, “mimics” the carbohydrate epitope Galα(1,3)Gal. In vitro studies demonstrated that the binding of the IB4 lectin or human natural antibodies to pig cells could be specifically blocked by Gal pep 1, in several different serological assays: a) the hemagglutnation of pig erythrocytes; b) cytofluorographic analysis of pig lymphocytes and endothelial cells; c) cytotoxicity of human serum against pig endothelial cells; d) an ELISA assay. The relative affinity of the peptide for the IB4 lectin was similar to that of α‐galactosyl sugars, although it was bound at a lower affinity by human antibodies. The implications of these observations to xenotransplantation are that peptides could theoretically be used to block hyperacute rejection.
Xenotransplantation – Wiley
Published: Feb 1, 1996
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