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Purification, crystallization and preliminary X‐ray diffraction studies of parakeet ( Psittacula krameri ) haemoglobin

Purification, crystallization and preliminary X‐ray diffraction studies of parakeet ( Psittacula... Birds often show efficient oxygen management in order to meet the special demands of their metabolism. However, the structural studies of avian haemoglobins (Hbs) are inadequate for complete understanding of the mechanism involved. Towards this end, purification, crystallization and preliminary X‐ray diffraction studies have been carried out for parakeet Hb. Parakeet Hb was crystallized as the met form in low‐salt buffered conditions after extracting haemoglobin from crude blood by microcentrifugation and purifying the sample by column chromatography. Good‐quality crystals were grown from 10% PEG 3350 and a crystal diffracted to about 2.8 Å resolution. Preliminary diffraction data showed that the Hb crystal belonged to the monoclinic system (space group C2), with unit‐cell parameters a = 110.68, b = 64.27, c = 56.40 Å, β = 109.35°. Matthews volume analysis indicated that the crystals contained a half‐tetramer in the asymmetric unit. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Purification, crystallization and preliminary X‐ray diffraction studies of parakeet ( Psittacula krameri ) haemoglobin

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References (10)

Publisher
Wiley
Copyright
International Union of Crystallography, 2009
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309109034071
pmid
19851014
Publisher site
See Article on Publisher Site

Abstract

Birds often show efficient oxygen management in order to meet the special demands of their metabolism. However, the structural studies of avian haemoglobins (Hbs) are inadequate for complete understanding of the mechanism involved. Towards this end, purification, crystallization and preliminary X‐ray diffraction studies have been carried out for parakeet Hb. Parakeet Hb was crystallized as the met form in low‐salt buffered conditions after extracting haemoglobin from crude blood by microcentrifugation and purifying the sample by column chromatography. Good‐quality crystals were grown from 10% PEG 3350 and a crystal diffracted to about 2.8 Å resolution. Preliminary diffraction data showed that the Hb crystal belonged to the monoclinic system (space group C2), with unit‐cell parameters a = 110.68, b = 64.27, c = 56.40 Å, β = 109.35°. Matthews volume analysis indicated that the crystals contained a half‐tetramer in the asymmetric unit.

Journal

Acta Crystallographica Section FWiley

Published: Oct 1, 2009

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