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- Publisher
- Wiley
- Copyright
- International Union of Crystallography, 2012
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309112030278
- pmid
- 22949205
- Publisher site
- See Article on Publisher Site
Abstract
Uracil‐DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N‐glycosidic bond, thereby initiating the base‐excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDGΔ) and stoUDGΔ complexed with uracil were crystallized and analyzed by X‐ray crystallography. The crystals were found to belong to the orthorhombic space group P212121, with unit‐cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDGΔ and stoUDGΔ complexed with uracil, respectively.
Journal
Acta Crystallographica Section F – Wiley
Published: Sep 1, 2012