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Purification, crystallization and preliminary crystallographic analysis of a 6‐pyruvoyltetrahydropterin synthase homologue from Esherichia coli

Purification, crystallization and preliminary crystallographic analysis of a... 6‐Pyruvoyltetrahydropterin synthase from E. coli (ePTPS) has been crystallized using the hanging‐drop vapour‐diffusion method. Hexagonal‐ and rectangular‐shaped crystals were obtained. Diffraction data were collected from the hexagonal and rectangular crystals to 3.0 and 2.3 Å resolution, respectively. The hexagonal plate‐shaped crystals belonged to space group P321, with unit‐cell parameters a = b = 112.59, c = 68.82 Å, and contained two molecules in the asymmetric unit. The rectangular crystals belonged to space group I222, with unit‐cell parameters a = 112.76, b = 117.66, c = 153.57 Å, and contained six molecules in the asymmetric unit. The structure of ePTPS in both crystal forms has been determined by molecular replacement. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Purification, crystallization and preliminary crystallographic analysis of a 6‐pyruvoyltetrahydropterin synthase homologue from Esherichia coli

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References (9)

Publisher
Wiley
Copyright
Copyright © 2008 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309108000626
Publisher site
See Article on Publisher Site

Abstract

6‐Pyruvoyltetrahydropterin synthase from E. coli (ePTPS) has been crystallized using the hanging‐drop vapour‐diffusion method. Hexagonal‐ and rectangular‐shaped crystals were obtained. Diffraction data were collected from the hexagonal and rectangular crystals to 3.0 and 2.3 Å resolution, respectively. The hexagonal plate‐shaped crystals belonged to space group P321, with unit‐cell parameters a = b = 112.59, c = 68.82 Å, and contained two molecules in the asymmetric unit. The rectangular crystals belonged to space group I222, with unit‐cell parameters a = 112.76, b = 117.66, c = 153.57 Å, and contained six molecules in the asymmetric unit. The structure of ePTPS in both crystal forms has been determined by molecular replacement.

Journal

Acta Crystallographica Section FWiley

Published: Feb 1, 2008

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