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- Publisher
- Wiley
- Copyright
- Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309107041176
- pmid
- 17909286
- Publisher site
- See Article on Publisher Site
Abstract
The peanut is a significant food source, but is responsible for many cases of anaphylaxis. The peanut 11S legumin‐like seed storage protein Ara h 3 is one of the best characterized allergens. In this study, Ara h 3 was extracted from peanut kernels and purified by sequential anion‐exchange, hydrophobic interaction and gel‐filtration chromatography to very high purity to facilitate crystallization and structural studies. Well diffracting single crystals were obtained by the vapor‐diffusion method. A molecular‐replacement structural solution has been obtained and refinement of the structure is currently under way.
Journal
Acta Crystallographica Section F – Wiley
Published: Oct 1, 2007