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Protein expression, crystallization and preliminary X‐ray crystallographic studies of LidA from Legionella pneumophila

Protein expression, crystallization and preliminary X‐ray crystallographic studies of LidA from... LidA, a translocated substrate of the Legionella pneumophila Dot/Icm type IV secretion system, is associated with maintenance of bacterial integrity and interferes with the early secretory pathway. However, the precise mechanism of LidA in these processes remains elusive. To further investigate the structure and function of LidA, the full‐length protein was successfully expressed in Escherichia coli and purified. LidA was crystallized using sitting‐drop vapour diffusion and diffracted to a resolution of 2.75 Å. The crystal belonged to space group P212121, with unit‐cell parameters a = 57.5, b = 64.5, c = 167.3 Å, α = β = γ = 90°. There is one molecule per asymmetric unit. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Protein expression, crystallization and preliminary X‐ray crystallographic studies of LidA from Legionella pneumophila

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References (12)

Publisher
Wiley
Copyright
International Union of Crystallography, 2011
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309111011286
pmid
21543880
Publisher site
See Article on Publisher Site

Abstract

LidA, a translocated substrate of the Legionella pneumophila Dot/Icm type IV secretion system, is associated with maintenance of bacterial integrity and interferes with the early secretory pathway. However, the precise mechanism of LidA in these processes remains elusive. To further investigate the structure and function of LidA, the full‐length protein was successfully expressed in Escherichia coli and purified. LidA was crystallized using sitting‐drop vapour diffusion and diffracted to a resolution of 2.75 Å. The crystal belonged to space group P212121, with unit‐cell parameters a = 57.5, b = 64.5, c = 167.3 Å, α = β = γ = 90°. There is one molecule per asymmetric unit.

Journal

Acta Crystallographica Section FWiley

Published: May 1, 2011

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