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Profiling Interactome Networks with the HaloTag‐NAPPA In Situ Protein Array

Profiling Interactome Networks with the HaloTag‐NAPPA In Situ Protein Array Physical interactions between proteins and other molecules can be evaluated at a proteome scale using protein arrays, a type of high‐throughput pulldown assay. We developed a modified in situ protein array known as the nucleic acid programmable protein assay (NAPPA) that allows the screening of thousands of open reading frames (ORFs) at a lower cost, with less labor, and in less time than conventional protein arrays. The HaloTag‐NAPPA protein array can efficiently capture proteins expressed in situ on a glass slide using the Halo high‐affinity capture tag. Here, we describe the fabrication of the array using publicly available resources and detection of protein‐protein interactions (PPIs) that can be used to generate a protein interactome map. The Basic Protocol includes procedures for preparing the plasmid DNA spotted on glass slides, in situ protein expression, and PPI detection. The supporting protocols outline the construction of vectors and preparation of ORF clones. © 2018 by John Wiley & Sons, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Protocols in Plant Biology Wiley

Profiling Interactome Networks with the HaloTag‐NAPPA In Situ Protein Array

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References (16)

Publisher
Wiley
Copyright
© 2018 John Wiley & Sons, Inc.
ISSN
2379-8068
eISSN
2379-8068
DOI
10.1002/cppb.20071
Publisher site
See Article on Publisher Site

Abstract

Physical interactions between proteins and other molecules can be evaluated at a proteome scale using protein arrays, a type of high‐throughput pulldown assay. We developed a modified in situ protein array known as the nucleic acid programmable protein assay (NAPPA) that allows the screening of thousands of open reading frames (ORFs) at a lower cost, with less labor, and in less time than conventional protein arrays. The HaloTag‐NAPPA protein array can efficiently capture proteins expressed in situ on a glass slide using the Halo high‐affinity capture tag. Here, we describe the fabrication of the array using publicly available resources and detection of protein‐protein interactions (PPIs) that can be used to generate a protein interactome map. The Basic Protocol includes procedures for preparing the plasmid DNA spotted on glass slides, in situ protein expression, and PPI detection. The supporting protocols outline the construction of vectors and preparation of ORF clones. © 2018 by John Wiley & Sons, Inc.

Journal

Current Protocols in Plant BiologyWiley

Published: Sep 1, 2018

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