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A cobalt hexacyanoferrate (CoHCF) nanoparticle (size ca. 60 nm) chemically modified electrode (CME) was fabricated and the electrochemical behavior of hemoglobin (Hb) at this nanosized CoHCF CME was studied. In comparison with a bare glassy carbon electrode (GCE) and a general CoHCF CME electrodeposited in a traditional manner, the present nanosized CoHCF CME performed efficiently electrocatalytic reduction for Hb with relatively high sensitivity, stability, and longlife. Combined with liquid chromatography (LC), the nanosized CoHCF CME was used as the electrochemical detector of Hb in the established flow injection analysis‐electrochemical determination (FIA‐ECD) system. The peak current was a linear function of concentrations in the range from 2.5×10‐8 to 5.0×10‐6 mol/L for Hb, with detection limit of 1.4×10‐8 mol/L. The FIA‐ECD system has been successfully applied to assess the Hb content of clinic blood samples with advantages of sensitiveness, speediness, easy control and small sample‐consumption.
Chinese Journal of Chemistry – Wiley
Published: Aug 1, 2005
Keywords: ; ; ; ;
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