Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Hartleib, H. Rüterjans (2001)
High-yield expression, purification, and characterization of the recombinant diisopropylfluorophosphatase from Loligo vulgaris.Protein expression and purification, 21 1
J. Habash, J. Raftery, S. Weisgerber, A. Cassetta, M. Lehmann, Peter Hghj, C. Wilkinson, J. Campbell, J. Helliwell (1997)
NEUTRON LAUE DIFFRACTION STUDY OF CONCANAVALIN A : THE PROTON OF ASP28Journal of the Chemical Society, Faraday Transactions, 93
A. Kossiakoff, S. Spencer (1981)
Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.Biochemistry, 20 22
M. Weiss, R. Hilgenfeld (2000)
Global Indicators of X-ray Data QualityActa Crystallographica Section A, 56
(2006)
Tetramer. ‡ Perdeuterated. § Dimer. Figure 3 Scatter plot of asymmetric unit volume versus crystal size, based on published neutron data (see Table 2). The solid line on the left represents the range
Vicky Katsemi, C. Lücke, J. Koepke, F. Löhr, S. Maurer, G. Fritzsch, H. Rüterjans (2005)
Mutational and structural studies of the diisopropylfluorophosphatase from Loligo vulgaris shed new light on the catalytic mechanism of the enzyme.Biochemistry, 44 25
(2001)
Biochemistry , 40 , 13149 – 13157 . Collaborative Computational Project , Number 4 ( 1994 )
M. Harel, A. Aharoni, L. Gaidukov, B. Brumshtein, O. Khersonsky, Ran Meged, H. Dvir, R. Ravelli, A. McCarthy, L. Toker, I. Silman, J. Sussman, Dan Tawfik (2004)
Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymesNature Structural &Molecular Biology, 11
M. Weiss, R. Hilgenfeld (1997)
On the use of the merging R factor as a quality indicator for X-ray dataJournal of Applied Crystallography, 30
P. Langan, Gayle Greene (2004)
Protein crystallography with spallation neutrons: collecting and processing wavelength‐resolved Laue protein dataJournal of Applied Crystallography, 37
L. Coates, P. Erskine, S. Wood, D. Myles, J. Cooper (2001)
A neutron Laue diffraction study of endothiapepsin: implications for the aspartic proteinase mechanism.Biochemistry, 40 44
J. Helliwell, J. Habash, D. Cruickshank, M. Harding, T. Greenhough, J. Campbell, I. Clifton, M. Elder, P. Machin, M. Papiz, S. Zurek (1989)
The recording and analysis of synchrotron X-radiation Laue diffraction photographsJournal of Applied Crystallography, 22
M. Blum, F. Löhr, A. Richardt, H. Rüterjans, J. Chen (2006)
Binding of a designed substrate analogue to diisopropyl fluorophosphatase: implications for the phosphotriesterase mechanism.Journal of the American Chemical Society, 128 39
B. Schoenborn (1969)
Neutron Diffraction Analysis of MyoglobinNature, 224
P. Langan, Gayle Greene, B. Schoenborn (2004)
Protein crystallography with spallation neutrons: the user facility at Los Alamos Neutron Science CenterJournal of Applied Crystallography, 37
A. Wlodawer, J. Walter, R. Huber, L. Sjölin (1984)
Structure of bovine pancreatic trypsin inhibitorJournal of Molecular Biology, 180
(1998)
New York: Plenum
The enzyme diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris is capable of decontaminating a wide variety of toxic organophosphorus nerve agents. DFPase is structurally related to a number of enzymes, such as the medically important paraoxonase (PON). In order to investigate the reaction mechanism of this phosphotriesterase and to elucidate the protonation state of the active‐site residues, large‐sized crystals of DFPase have been prepared for neutron diffraction studies. Available H atoms have been exchanged through vapour diffusion against D2O‐containing mother liquor in the capillary. A neutron data set has been collected to 2.2 Å resolution on a relatively small (0.43 mm3) crystal at the spallation source in Los Alamos. The sample size and asymmetric unit requirements for the feasibility of neutron diffraction studies are summarized.
Acta Crystallographica Section F – Wiley
Published: Jan 1, 2007
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.