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Preliminary crystallographic characterization of PrnB, the second enzyme in the pyrrolnitrin biosynthetic pathway

Preliminary crystallographic characterization of PrnB, the second enzyme in the pyrrolnitrin... Pyrrolnitrin is the active ingredient of drugs for the treatment of superficial fungal infections and was used as a lead structure for the development of fludioxonil. It is an effective agent for plant diseases caused by the fungal pathogen Rhizoctonia solani. Pyrrolnitrin is made in four steps, the second of which, catalyzed by PrnB, is a novel chemical rearrangement of 7‐chlorotryptophan. PrnB was overproduced in Pseudomonas fluorescens (BL915) and well diffracting crystals were obtained of a triple cysteine‐to‐serine mutant by sitting‐drop vapour diffusion. Crystals grown in the presence of l‐7‐chlorotryptophan, d‐­tryptophan and l‐tryptophan are reported. Data sets for each are reported with high‐resolution limits of 2.0, 1.75 and 1.75 Å, respectively. Two crystals (PrnB in the presence of d‐tryptophan and l‐7‐chlorotryptophan) belong to space group C2 with similar unit‐cell parameters (a = 68.6, b = 79.5, c = 92.7 Å, α = γ = 90.0, β = 103.8°). Crystals grown in the presence of l‐­tryptophan belong to space group C2221 and have unit‐cell parameters a = 67.7, b = 80.1, c = 129.5 Å. All crystals contain a monomer in the asymmetric unit. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Preliminary crystallographic characterization of PrnB, the second enzyme in the pyrrolnitrin biosynthetic pathway

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References (33)

Publisher
Wiley
Copyright
Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309106041649
pmid
17077497
Publisher site
See Article on Publisher Site

Abstract

Pyrrolnitrin is the active ingredient of drugs for the treatment of superficial fungal infections and was used as a lead structure for the development of fludioxonil. It is an effective agent for plant diseases caused by the fungal pathogen Rhizoctonia solani. Pyrrolnitrin is made in four steps, the second of which, catalyzed by PrnB, is a novel chemical rearrangement of 7‐chlorotryptophan. PrnB was overproduced in Pseudomonas fluorescens (BL915) and well diffracting crystals were obtained of a triple cysteine‐to‐serine mutant by sitting‐drop vapour diffusion. Crystals grown in the presence of l‐7‐chlorotryptophan, d‐­tryptophan and l‐tryptophan are reported. Data sets for each are reported with high‐resolution limits of 2.0, 1.75 and 1.75 Å, respectively. Two crystals (PrnB in the presence of d‐tryptophan and l‐7‐chlorotryptophan) belong to space group C2 with similar unit‐cell parameters (a = 68.6, b = 79.5, c = 92.7 Å, α = γ = 90.0, β = 103.8°). Crystals grown in the presence of l‐­tryptophan belong to space group C2221 and have unit‐cell parameters a = 67.7, b = 80.1, c = 129.5 Å. All crystals contain a monomer in the asymmetric unit.

Journal

Acta Crystallographica Section FWiley

Published: Nov 1, 2006

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