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N. Kirchhof, S. Shibata, M. Wijkstrom, D. Kulick, C. Salerno, S. Clemmings, Y. Heremans, U. Galili, D. Sutherland, A. Dalmasso, B. Hering (2004)
Reversal of diabetes in non‐immunosuppressed rhesus macaques by intraportal porcine islet xenografts precedes acute cellular rejectionXenotransplantation, 11
J. Söderlund, L. Wennberg, E. Castaños-Vélez, P. Biberfeld, S. Zhu, A. Tibell, C. Groth, O. Korsgren (1999)
Fetal porcine islet-like cell clusters transplanted to cynomolgus monkeys: an immunohistochemical study.Transplantation, 67 6
B. Schröppel, N. Zhang, Peng Chen, W. Zang, Dongmei Chen, K. Hudkins, W. Kuziel, R. Sung, J. Bromberg, B. Murphy (2004)
Differential expression of chemokines and chemokine receptors in murine islet allografts: the role of CCR2 and CCR5 signaling pathways.Journal of the American Society of Nephrology : JASN, 15 7
(1997)
Relative quantification of gene expression. ABI 7700 Sequence Detection System
R. Abdi, T. Means, Toshiro Ito, R. Smith, N. Najafian, Mollie Jurewicz, Vaja Tchipachvili, I. Charo, H. Auchincloss, M. Sayegh, A. Luster (2004)
Differential Role of CCR2 in Islet and Heart Allograft Rejection: Tissue Specificity of Chemokine/Chemokine Receptor Function In Vivo1The Journal of Immunology, 172
C. Ramakers, J. Ruijter, R. Deprez, A. Moorman (2003)
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) dataNeuroscience Letters, 339
R. Abdi, R. Smith, Leila Makhlouf, N. Najafian, A. Luster, H. Auchincloss, M. Sayegh (2002)
The role of CC chemokine receptor 5 (CCR5) in islet allograft rejection.Diabetes, 51 8
Heike Unsoeld, S. Krautwald, D. Voehringer, U. Kunzendorf, H. Pircher (2002)
Cutting Edge: CCR7+ and CCR7− Memory T Cells Do Not Differ in Immediate Effector Cell Function1The Journal of Immunology, 169
N. Inston, P. Cockwell (2002)
The evolving role of chemokines and their receptors in acute allograft rejection.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 17 8
R. Abdi, T. Means, A. Luster (2003)
Chemokines in islet allograft rejectionDiabetes/Metabolism Research and Reviews, 19
M. Eisen, P. Spellman, P. Brown, D. Botstein (1998)
Cluster analysis and display of genome-wide expression patterns.Proceedings of the National Academy of Sciences of the United States of America, 95 25
W. Gao, P. Topham, J. King, S. Smiley, V. Csizmadia, B. Lu, C. Gerard, W. Hancock (2000)
Targeting of the chemokine receptor CCR1 suppresses development of acute and chronic cardiac allograft rejection.The Journal of clinical investigation, 105 1
P. Schmidt, H. Krook, A. Maeda, O. Korsgren, B. Benda (2003)
A new murine model of islet xenograft rejection: graft destruction is dependent on a major histocompatibility-specific interaction between T-cells and macrophages.Diabetes, 52 5
M. Paule, M. Paule, Shaun McColl, Shaun McColl, C. Simeonovic, C. Simeonovic (2000)
Murine chemokine gene expression in rejecting pig proislet xenografts.Transplantation proceedings, 32 5
H. Krook, A. Hagberg, Zhenshun Song, U. Landegren, L. Wennberg, O. Korsgren (2002)
A distinct Th1 immune response precedes the described Th2 response in islet xenograft rejection.Diabetes, 51 1
David Zhao, Yenya Hu, G. Miller, A. Luster, R. Mitchell, P. Libby (2002)
Differential Expression of the IFN-γ-Inducible CXCR3-Binding Chemokines, IFN-Inducible Protein 10, Monokine Induced by IFN, and IFN-Inducible T Cell α Chemoattractant in Human Cardiac Allografts: Association with Cardiac Allograft Vasculopathy and Acute Rejection1The Journal of Immunology, 169
S. Yi, Ximin Feng, W. Hawthorne, Anita Patel, S. Walters, P. O’Connell (2000)
CD8+ T cells are capable of rejecting pancreatic islet xenografts.Transplantation, 70 6
T. el-Sawy, Nader Fahmy, R. Fairchild (2002)
Chemokines: directing leukocyte infiltration into allografts.Current opinion in immunology, 14 5
F. Sallusto, D. Lenig, R. Förster, M. Lipp, A. Lanzavecchia (1999)
Two subsets of memory T lymphocytes with distinct homing potentials and effector functionsNature, 401
Abdi Abdi, Smith Smith, Makhlouf Makhlouf (5)
(CCR5) in islet allograft rejectionDiabetes, 2002
M. Baker, Xiaojuan Chen, A. Rotramel, J. Nelson, B. Lu, C. Gerard, Y. Kanwar, D. Kaufman (2003)
Genetic deletion of chemokine receptor CXCR3 or antibody blockade of its ligand IP-10 modulates posttransplantation graft-site lymphocytic infiltrates and prolongs functional graft survival in pancreatic islet allograft recipients.Surgery, 134 2
P. Murphy, M. Baggiolini, I. Charo, C. Hébert, R. Horuk, K. Matsushima, L. Miller, J. Oppenheim, C. Power (2000)
International union of pharmacology. XXII. Nomenclature for chemokine receptors.Pharmacological reviews, 52 1
Iris Lee, Liqing Wang, A. Wells, Qunrui Ye, R. Han, M. Dorf, W. Kuziel, B. Rollins, Lieping Chen, W. Hancock (2003)
Blocking the Monocyte Chemoattractant Protein-1/CCR2 Chemokine Pathway Induces Permanent Survival of Islet Allografts through a Programmed Death-1 Ligand-1-Dependent Mechanism 1The Journal of Immunology, 171
M. Solomon, W. Kuziel, D. Mann, C. Simeonovic (2003)
The role of chemokines and their receptors in the rejection of pig islet tissue xenograftsXenotransplantation, 10
T. Olson, K. Ley (2002)
Chemokines and chemokine receptors in leukocyte trafficking.American journal of physiology. Regulatory, integrative and comparative physiology, 283 1
S. Yi, Ximin Feng, Yiping Wang, Thomas Kay, Yao Wang, P. O’Connell (1999)
CD4+ cells play a major role in xenogeneic human anti-pig cytotoxicity through the Fas/Fas ligand lytic pathway.Transplantation, 67 3
W. Hancock, B. Lu, Wei Gao, V. Csizmadia, K. Faia, J. King, S. Smiley, Mai Ling, N. Gerard, C. Gerard (2000)
Requirement of the Chemokine Receptor CXCR3 for Acute Allograft RejectionThe Journal of Experimental Medicine, 192
Wayne Hancock, Wei Gao, K. Faia, V. Csizmadia (2000)
Chemokines and their receptors in allograft rejection.Current opinion in immunology, 12 5
F. Sallusto, A. Lanzavecchia (2000)
Understanding dendritic cell and T‐lymphocyte traffic through the analysis of chemokine receptor expressionImmunological Reviews, 177
C. Agostini, F. Calabrese, F. Rea, M. Facco, A. Tosoni, M. Loy, G. Binotto, M. Valente, L. Trentin, G. Semenzato (2001)
Cxcr3 and its ligand CXCL10 are expressed by inflammatory cells infiltrating lung allografts and mediate chemotaxis of T cells at sites of rejection.The American journal of pathology, 158 5
D. Cantarovich, G. Blancho, N. Potiron, N. Jugeau, M. Fiche, C. Chagneau, E. Letessier, F. Boeffard, P. Loth, G. Karam, J. Soulillou, B. Mauff (2002)
Rapid failure of pig islet transplantation in non human primatesXenotransplantation, 9
T. Bar, A. Ståhlberg, Anders Muszta, M. Kubista (2003)
Kinetic Outlier Detection (KOD) in real-time PCR.Nucleic acids research, 31 17
L. Bühler, S. Deng, J. O'neil, H. Kitamura, M. Koulmanda, A. Baldi, J. Rahier, I. Alwayn, J. Appel, M. Awwad, D. Sachs, G. Weir, J. Squifflet, D. Cooper, P. Morel (2002)
Adult porcine islet transplantation in baboons treated with conventional immunosuppression or a non‐myeloablative regimen and CD154 blockadeXenotransplantation, 9
Abstract: Background: We have previously shown that pig‐to‐primate intraportal islet xenografts reverse diabetes, escape hyperacute rejection, and undergo acute cellular rejection in non‐immunosuppressed recipients. To gain a better understanding of mechanisms contributing to xenoislet rejection in non‐human primates we examined gene expression in livers bearing islet xenografts in the first 72 h after transplantation. Methods: Liver specimens were collected at sacrifice from seven non‐immunosuppressed rhesus macaques at 12, 24, 48 and 72 h after intraportal porcine islet transplantation. Following total RNA extraction, mRNA was quantified using SYBR green real‐time reverse transcription polymerase chain reaction (RT‐PCR) for species‐specific immune response genes. Data were analyzed using comparative cycle threshold (Ct) analysis, adjusted for specific primer‐efficiencies and normalized to cyclophilin expression. Results: Porcine insulin mRNA was detected in all liver samples. Cluster analysis revealed differential gene expression patterns at 12 and 24 h (early) compared with at 48 and 72 h (late) post‐transplant. Gene expression patterns were associated with histological findings of predominantly neutrophils and only a few lymphocytes at 12 and 24 h and an increasing number of lymphocytes and macrophages at 48 and 72 h. Transcript levels of CXCR3 and its ligands, interferon‐inducible protein 10 (IP‐10) and monokine induced by IFN‐γ (Mig), significantly increased between early and late time points together with expression of MIP‐1α, regulated on activation normal T expressed and secreted protein (RANTES) and MCP‐1. CCR5 showed only a marginal, non‐significant increase. Fas ligand, perforin and granzyme B transcripts were all elevated at 48 and 72 h post‐transplant. Conclusions: Our data suggest that CXCR3, with ligands IP‐10 and Mig, is involved in T cell recruitment in acute islet xenograft rejection in non‐human primates. Upregulation of RANTES and MIP‐1α transcripts in the absence of a significant CCR5 increase suggests a possible involvement of other chemokine receptors. MCP‐1 expression is associated with T cell and macrophage infiltration. Elevated cytotoxic effector molecule expression (Fas ligand, perforin, granzyme B) indicates T‐cell mediated graft destruction by cytotoxic and cytolytic mechanisms within 48 to 72 h after transplantation. These results identify the CXCR3‐mediated chemoattractant pathway as an immunosuppressive target in pig‐to‐primate islet xenotransplantation.
Xenotransplantation – Wiley
Published: Jul 1, 2005
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