Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Peptide‐Conjugated Silver Nanoparticles for the Colorimetric Detection of the Oncoprotein Mdm2 in Human Serum

Peptide‐Conjugated Silver Nanoparticles for the Colorimetric Detection of the Oncoprotein Mdm2 in... The development of efficient, reliable, and easy‐to‐use biosensors allowing early cancer diagnosis is of paramount importance for patients. Herein, we report a biosensor based on silver nanoparticles functionalized by peptide aptamers for the detection of a cancer biomarker, i. e. the Mdm2 protein. Silver nanoparticles (AgNPs) were produced and stabilized with a thin PEGylated‐calix[4]arene layer that allows (i) the steric stabilization of the AgNPs and (ii) the covalent conjugation of the peptide aptamers via the formation of an amide bond. These peptide‐conjugated AgNPs were then used to detect Mdm2 via a dual trapping strategy that was previously reported with gold nanoparticles (AuNPs). Our results showed that replacing AuNPs by AgNPs improves the detection limit by nearly one order of magnitude, down to 5 nM, while the high selectivity of the system and the stability of the particles provided by the calixarene coating allow the detection of Mdm2 in human serum. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png ChemPlusChem Wiley

Peptide‐Conjugated Silver Nanoparticles for the Colorimetric Detection of the Oncoprotein Mdm2 in Human Serum

Loading next page...
 
/lp/wiley/peptide-conjugated-silver-nanoparticles-for-the-colorimetric-detection-zmeaMFs2pg

References (0)

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Wiley
Copyright
© 2022 Wiley‐VCH GmbH
eISSN
2192-6506
DOI
10.1002/cplu.202100450
Publisher site
See Article on Publisher Site

Abstract

The development of efficient, reliable, and easy‐to‐use biosensors allowing early cancer diagnosis is of paramount importance for patients. Herein, we report a biosensor based on silver nanoparticles functionalized by peptide aptamers for the detection of a cancer biomarker, i. e. the Mdm2 protein. Silver nanoparticles (AgNPs) were produced and stabilized with a thin PEGylated‐calix[4]arene layer that allows (i) the steric stabilization of the AgNPs and (ii) the covalent conjugation of the peptide aptamers via the formation of an amide bond. These peptide‐conjugated AgNPs were then used to detect Mdm2 via a dual trapping strategy that was previously reported with gold nanoparticles (AuNPs). Our results showed that replacing AuNPs by AgNPs improves the detection limit by nearly one order of magnitude, down to 5 nM, while the high selectivity of the system and the stability of the particles provided by the calixarene coating allow the detection of Mdm2 in human serum.

Journal

ChemPlusChemWiley

Published: Apr 1, 2022

Keywords: calixarenes; colorimetric detection; oncoproteins; peptide aptamers; silver nanoparticles

There are no references for this article.