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Overproduction, purification and preliminary X‐ray diffraction analysis of CzcE from Cupriavidus metallidurans CH34

Overproduction, purification and preliminary X‐ray diffraction analysis of CzcE from Cupriavidus... CzcE is encoded by the czc determinant that allows Cupriavidus metallidurans CH34 to modulate its internal concentrations of cobalt, zinc and cadmium. This periplasmic protein was overproduced in its mature form in Escherichia coli and purified in two steps. After preliminary screening of crystallization conditions using a robot, well diffracting crystals were obtained using the hanging‐drop vapour‐diffusion method. Crystals diffracted to 1.96 Å using synchrotron radiation. They belonged to the monoclinic space group C2, with unit‐cell parameters a = 105.54, b = 29.68, c = 71.10 Å. The asymmetric unit is expected to contain a dimer, in agreement with the quaternary structure deduced from gel‐filtration experiments. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Overproduction, purification and preliminary X‐ray diffraction analysis of CzcE from Cupriavidus metallidurans CH34

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References (9)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309107043709
pmid
17909296
Publisher site
See Article on Publisher Site

Abstract

CzcE is encoded by the czc determinant that allows Cupriavidus metallidurans CH34 to modulate its internal concentrations of cobalt, zinc and cadmium. This periplasmic protein was overproduced in its mature form in Escherichia coli and purified in two steps. After preliminary screening of crystallization conditions using a robot, well diffracting crystals were obtained using the hanging‐drop vapour‐diffusion method. Crystals diffracted to 1.96 Å using synchrotron radiation. They belonged to the monoclinic space group C2, with unit‐cell parameters a = 105.54, b = 29.68, c = 71.10 Å. The asymmetric unit is expected to contain a dimer, in agreement with the quaternary structure deduced from gel‐filtration experiments.

Journal

Acta Crystallographica Section FWiley

Published: Oct 1, 2007

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