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Oral Communication: Basic

Oral Communication: Basic 166 Peripheral B cell culture for assessment of immune response to ABO blood type carbohydrate antigens Masataka Haneda 1 , Yoshiko Matsuda 1 , Yuko Miwa 1 , Kenta Iwasaki 1 , Yoshihiko Watarai 2 , Kazuharu Uchida 3 , Takaaki Kobayashi 1 1 Transplant Immunology, Nagoya University Graduate School of Medicine, Nagoya; 2 Transplant and Endocrine Surgery, Nagoya Daini Red Cross Hospital, Nagoya; 3 Organ Transplant Surgery, Aichi Medical University, Nagakute, Japan By introduction of the desensitization therapy including rituximab, ABO blood group incompatible (ABO‐I) kidney transplantation has been well‐established as an effective treatment, despite the presence of anti‐donor antibody [1]. Although most transplants showed favorable outcome, probably due to accommodation, some exhibited severe antibody mediated rejection (AMR) by anti‐donor A/B antibody, leading to worsening post‐transplant results. Here we report in vitro anti‐A/B antibody production system using B cell culture, which can induce cell differentiation from mature to plasma cells from peripheral blood B cells in vitro. Human B cells were isolated from PBMCs of healthy human volunteers by positive selection of cells expressing CD19 antigen using MACS separation. B cells were cultured in IMDM containing 10% FBS, supplemented with 50 ug/ml of human transferrin‐selenium, 5 ug/ml http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Oral Communication: Basic

Xenotransplantation , Volume 20 (5) – Sep 1, 2013
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Publisher
Wiley
Copyright
© 2013 John Wiley & Sons A/S
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/xen.12060_3
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Abstract

166 Peripheral B cell culture for assessment of immune response to ABO blood type carbohydrate antigens Masataka Haneda 1 , Yoshiko Matsuda 1 , Yuko Miwa 1 , Kenta Iwasaki 1 , Yoshihiko Watarai 2 , Kazuharu Uchida 3 , Takaaki Kobayashi 1 1 Transplant Immunology, Nagoya University Graduate School of Medicine, Nagoya; 2 Transplant and Endocrine Surgery, Nagoya Daini Red Cross Hospital, Nagoya; 3 Organ Transplant Surgery, Aichi Medical University, Nagakute, Japan By introduction of the desensitization therapy including rituximab, ABO blood group incompatible (ABO‐I) kidney transplantation has been well‐established as an effective treatment, despite the presence of anti‐donor antibody [1]. Although most transplants showed favorable outcome, probably due to accommodation, some exhibited severe antibody mediated rejection (AMR) by anti‐donor A/B antibody, leading to worsening post‐transplant results. Here we report in vitro anti‐A/B antibody production system using B cell culture, which can induce cell differentiation from mature to plasma cells from peripheral blood B cells in vitro. Human B cells were isolated from PBMCs of healthy human volunteers by positive selection of cells expressing CD19 antigen using MACS separation. B cells were cultured in IMDM containing 10% FBS, supplemented with 50 ug/ml of human transferrin‐selenium, 5 ug/ml

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Xenotransplantation – Wiley

Published: Sep 1, 2013

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