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Online SERS Quantification of Staphylococcus aureus and the Application to Diagnostics in Human Fluids

Online SERS Quantification of Staphylococcus aureus and the Application to Diagnostics in Human... Staphylococcus aureus is a common cause of serious infections. One of the main drawbacks in its treatment is the time required for a positive diagnosis, over 24 h, as most methods are still based in bacterial culture. Herein, a microfluidic optical device for the rapid and ultrasensitive quantification of S. aureus in real human fluids is designed. In this approach, the surface‐enhanced Raman scattering (SERS)‐encoded particles, functionalized with either an antibody or an aptamer, form a dense collection of electromagnetic hot spots on the surface of S. aureus. This allows for an exponentially increase of the SERS signal when particles accumulate on the microorganism as compared to their free condition in bulk solution. Quantification is achieved by passing the sample through a microfluidic device with a collection window where a laser interrogates and classifies each of the induced bacteria–nanoparticle aggregates in real time. Further, the advantages of using aptamers versus antibodies as biorecognition elements are extensively investigated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Advanced Materials Technologies Wiley

Online SERS Quantification of Staphylococcus aureus and the Application to Diagnostics in Human Fluids

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References (47)

Publisher
Wiley
Copyright
Copyright © 2016 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
eISSN
2365-709X
DOI
10.1002/admt.201600163
Publisher site
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Abstract

Staphylococcus aureus is a common cause of serious infections. One of the main drawbacks in its treatment is the time required for a positive diagnosis, over 24 h, as most methods are still based in bacterial culture. Herein, a microfluidic optical device for the rapid and ultrasensitive quantification of S. aureus in real human fluids is designed. In this approach, the surface‐enhanced Raman scattering (SERS)‐encoded particles, functionalized with either an antibody or an aptamer, form a dense collection of electromagnetic hot spots on the surface of S. aureus. This allows for an exponentially increase of the SERS signal when particles accumulate on the microorganism as compared to their free condition in bulk solution. Quantification is achieved by passing the sample through a microfluidic device with a collection window where a laser interrogates and classifies each of the induced bacteria–nanoparticle aggregates in real time. Further, the advantages of using aptamers versus antibodies as biorecognition elements are extensively investigated.

Journal

Advanced Materials Technologies – Wiley

Published: Nov 1, 2016

Keywords: ; ; ;

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