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Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase

Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human... Summary Glutamic acid decarboxylase (GAD) catalyses the conversion of l‐glutamic acid to the inhibitory neurotransmitter γ‐aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. A full length human GAD67 cDNA has been isolated from a human frontal cortex cDNA library and the nucleotide sequence determined. The GAD67 gene has been mapped to chromosome 2 using the polymerase chain reaction to amplify specifically the human sequence in rodent/human somatic cell hybrid DNA. This confirms that human GAD67 is not syntenic with the smaller GAD isoform GAD65 which has been assigned to chromosome 10. Production of polyclonal antiserum to a baculovirus‐expressed GAD67 enabled immunocytological detection of GAD in the rat brain. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Human Genetics Wiley

Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase

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References (37)

Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0003-4800
eISSN
1469-1809
DOI
10.1111/j.1469-1809.1992.tb01150.x
Publisher site
See Article on Publisher Site

Abstract

Summary Glutamic acid decarboxylase (GAD) catalyses the conversion of l‐glutamic acid to the inhibitory neurotransmitter γ‐aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. A full length human GAD67 cDNA has been isolated from a human frontal cortex cDNA library and the nucleotide sequence determined. The GAD67 gene has been mapped to chromosome 2 using the polymerase chain reaction to amplify specifically the human sequence in rodent/human somatic cell hybrid DNA. This confirms that human GAD67 is not syntenic with the smaller GAD isoform GAD65 which has been assigned to chromosome 10. Production of polyclonal antiserum to a baculovirus‐expressed GAD67 enabled immunocytological detection of GAD in the rat brain.

Journal

Annals of Human GeneticsWiley

Published: Jul 1, 1992

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