Access the full text.
Sign up today, get DeepDyve free for 14 days.
Jay Fishman (1997)
Xenosis and xenotransplantation: addressing the infectious risks posed by an emerging technology.Kidney international. Supplement, 58
Goodwin Ah, D. Cooper, A. Malcolm, Ippolito Rm, E. Koren, F. Neethling, Y. Ye, N. Zuhdi, Lamontagne Lr (1992)
Identification of carbohydrate structures that bind human antiporcine antibodies: implications for discordant xenografting in humans.Transplantation proceedings, 24 2
R Larsen, Valanila Rajan, Melissa Ruff, J. Kukowska-Latallo, R Cummings, John Lowe (1989)
Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer.Proceedings of the National Academy of Sciences of the United States of America, 86 21
M. Basu, S. Basu (1973)
Enzymatic synthesis of a blood group B-related pentaglycosylceramide by an alpha-galactosyltransferase from rabbit bone marrow.The Journal of biological chemistry, 248 5
Robert, D. Larsen, Carlos, A., Rivera-Marrerot, Linda, K., Ernst, Richard, D. CummingsQ, John, B. Lowe (2001)
Frameshift and Nonsense Mutations in a Human Genomic Sequence Homologous to a Murine UDP-Gal:&D-Gal( 1,4)-D-GlcNAc CY( 1,3)-Galactosyltransferase cDNA*
D. Joziasse, J. Shaper, D. Eijnden, A. Tunen, N. Shaper (1989)
Bovine alpha 1----3-galactosyltransferase: isolation and characterization of a cDNA clone. Identification of homologous sequences in human genomic DNA.The Journal of biological chemistry, 264 24
U. Martin, V. Kiessig, J. Blusch, A. Haverich, Prof. Steinhoff (1998)
Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cellsThe Lancet, 352
D. Cooper, Goodwin Ah, E. Koren, R. Oriol, Malcolm Aj, Ippolito Rm, F. Neethling, Y. Ye, E. Romano, N. Zuhdi (1993)
Identification of alpha-galactosyl and other carbohydrate epitopes that are bound by human anti-pig antibodies: relevance to discordant xenografting in man.Transplant immunology, 1 3
David Onions, D. Cooper, Tom Alexander, Corrie Brown, Erwin Claassen, J. Foweraker, Delbert Harris, B. Mahy, Phillip Minor, A. Osterhaus, Paul-Pierre Pastoret, Kazuya Yamanouchi (2000)
An approach to the control of disease transmission in pig‐to‐human xenotransplantationXenotransplantation, 7
B. Morgan (1995)
Physiology and pathophysiology of complement: progress and trends.Critical reviews in clinical laboratory sciences, 32 3
C. Phelps, C. Koike, T. Vaught, J. Boone, K. Wells, Shu-Hung Chen, S. Ball, S. Specht, I. Polejaeva, Jeff Monahan, P. Jobst, Sugandha Sharma, Ashley Lamborn, A. Garst, M. Moore, A. Demetris, W. Rudert, R. Bottino, S. Bertera, M. Trucco, T. Starzl, Yifan Dai, D. Ayares (2003)
Production of alpha 1,3-galactosyltransferase-deficient pigs.Science, 299 5605
Spiro Spiro, Bhoyroo Bhoyroo (1984)
Occurrence of α ‐D‐galactosyl residues in the thyroglobulins from several speciesJ Biol Chem, 259
L. Chapman, T. Folks, D. Salomon, Amy Patterson, T. Eggerman, P. Noguchi (1995)
Xenotransplantation and xenogeneic infections.The New England journal of medicine, 333 22
Carol Phelps, Chihiro Koike, T. Vaught, J. Boone, Kevin Wells, Shu-Hung Chen, S. Ball, S. Specht, I. Polejaeva, Jeff Monahan, P. Jobst, Sugandha Sharma, Ashley Lamborn, A. Garst, Marilyn Moore, A. Demetris, W. Rudert, R. Bottino, S. Bertera, Massimo Trucco, Thomas Starzl, Yifan Dai, D. Ayares (2002)
Production of α1,3-Galactosyltransferase-Deficient PigsScience, 299
U. Galili (1993)
Interaction of the natural anti-Gal antibody with alpha-galactosyl epitopes: a major obstacle for xenotransplantation in humans.Immunology today, 14 10
Y. Takeuchi, C. Porter, K. Strahan, A. Preece, K. Gustafsson, F. Cosset, R. Weiss, M. Collins (1996)
Sensitization of cells and retroviruses to human serum by (αl-3) galactosyltransferaseNature, 379
T. Henion, B. Macher, F. Anaraki, U. Galili (1994)
Defining the minimal size of catalytically active primate alpha 1,3 galactosyltransferase: structure-function studies on the recombinant truncated enzyme.Glycobiology, 4 2
U. Galili, R. Mandrell, Randa Hamadeh, S. Shohet, J. Griffiss (1988)
Interaction between human natural anti-alpha-galactosyl immunoglobulin G and bacteria of the human floraInfection and Immunity, 56
R. Weiss (1998)
Transgenic pigs and virus adaptationNature, 391
T. Starzl, T. Marchioro, D. Talmage, W. Waddell (1963)
Splenectomy and Thymectomy in Human Renal Homotransplantation.∗Proceedings of the Society for Experimental Biology and Medicine, 113
T. Deacon, J. Schumacher, J. Dinsmore, Christine Thomas, P. Palmer, Stephen Kott, A. Edge, D. Penney, S. Kassissieh, P. Dempsey, O. Isacson (1997)
Histological evidence of fetal pig neural cell survival after transplantation into a patient with Parkinson's diseaseNature Medicine, 3
C. Groth, A. Tibell, J. Tollemar, J. Bolinder, J. Östman, E. Möller, F. Reinholt, O. Korsgren, C. Hellerström, A. Andersson (1994)
Transplantation of porcine fetal pancreas to diabetic patientsThe Lancet, 344
S. Tomlinson (1993)
Complement defense mechanisms.Current opinion in immunology, 5 1
Brice McKane, S. Ramachandran, Junbao Yang, Xìao-chun Xu, T. Mohanakumar (2003)
Xenoreactive anti-Galalpha(1,3)Gal antibodies prevent porcine endogenous retrovirus infection of human in vivo.Human immunology, 64 7
R. Spiro, V. Bhoyroo (1984)
Occurrence of alpha-D-galactosyl residues in the thyroglobulins from several species. Localization in the saccharide chains of the complex carbohydrate units.The Journal of biological chemistry, 259 15
D. Fine (1977)
Comparison of ethyleneglycoltetraacetic acid and its magnesium salt as reagents for studying alternative complement pathway functionInfection and Immunity, 16
Y. Takeuchi, SHIH-HUI Liong, P. Bieniasz, U. Jäger, C. Porter, T. Friedmann, M. Mcclure, R. Weiss (1997)
Sensitization of rhabdo-, lenti-, and spumaviruses to human serum by galactosyl(alpha1-3)galactosylationJournal of Virology, 71
Alan Betteridge, Winifred Watkins (1983)
Two alpha-3-D-galactosyltransferases in rabbit stomach mucosa with different acceptor substrate specificities.European journal of biochemistry, 132 1
Keith Reemtsma, Brian Mccracken, Jorgen Schlegel, Robert Hewitt, R. Flinner, Oscar Creech, Maurice Pearl, Charles Pearce, Charles DeWitt, Prentiss Smith (1964)
RENAL HETEROTRANSPLANTATION IN MAN.Annals of surgery, 160
L. Lai, D. Kolber-Simonds, Kwang‐Wook Park, H. Cheong, J. Greenstein, G. Im, M. Samuel, A. Bonk, A. Rieke, B. Day, C. Murphy, D. Carter, R. Hawley, R. Prather (2002)
Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer CloningScience, 295
G. Quinn, James Wood, D. Ryan, K. Suling, K. Moran, D. Kolber-Simonds, J. Greenstein, H. Schuurman, R. Hawley, C. Patience (2004)
Porcine Endogenous Retrovirus Transmission Characteristics of Galactose α1-3 Galactose-Deficient Pig CellsJournal of Virology, 78
D. Joziasse, J. Shaper, E. Jabs, N. Shaper (1991)
Characterization of an alpha 1----3-galactosyltransferase homologue on human chromosome 12 that is organized as a processed pseudogene.The Journal of biological chemistry, 266 11
R. Bryant, David Jenkins (1968)
Calcium requirements for complement dependent hemolytic reactions.Journal of immunology, 101 4
J. Platt (1998)
New directions for organ transplantation.Nature, 392 6679 Suppl
R. Oriol, Y. Ye, E. Koren, D. Cooper (1993)
CARBOHYDRATE ANTIGENS OF PIG TISSUES REACTING WITH HUMAN NATURAL ANTIBODIES AS POTENTIAL TARGETS FOR HYPERACUTE VASCULAR REJECTION IN PIG‐TO‐MAN ORGAN XENOTRANSPLANTATION1Transplantation, 56
U. Galili, S. Shohet, E. Kobrin, C. Stults, B. Macher (1988)
Man, apes, and Old World monkeys differ from other mammals in the expression of alpha-galactosyl epitopes on nucleated cells.The Journal of biological chemistry, 263 33
J. Fishman (1994)
Miniature swine as organ donors for man: Strategies for prevention of xenotransplant‐associated infectionsXenotransplantation, 1
D. Hunkeler, A. Sun, G. Korbutt, R. Rajotte, R. Gill, R. Calafiore, P. Morel (1999)
Bioartificial organs and acceptable riskNature Biotechnology, 17
Y. Takeuchi, C. Porter, K. Strahan, A. Preece, K. Gustafsson, F. Cosset, R. Weiss, M. Collins (1996)
Sensitization of cells and retroviruses to human serum by (alpha 1-3) galactosyltransferase.Nature, 379 6560
W. Vine, A. Kier, T. Starzl, V. Warty (1993)
Baboon-to-human liver transplantationThe Lancet, 341
N. Cooper (1991)
Complement evasion strategies of microorganisms.Immunology today, 12 9
C. Wood, C. Wood, E. Kabat, E. Kabat, L. Murphy, L. Murphy, I. Goldstein, I. Goldstein (1979)
Immunochemical studies of the combining sites of the two isolectins, A4 and B4, isolated from Bandeiraea simplicifolia.Archives of biochemistry and biophysics, 198 1
D. Joziasse (1992)
Mammalian glycosyltransferases: genomic organization and protein structure.Glycobiology, 2 4
S. Magre, Y. Takeuchi, G. Langford, A. Richards, C. Patience, R. Weiss (2004)
Reduced Sensitivity to Human Serum Inactivation of Enveloped Viruses Produced by Pig Cells Transgenic for Human CD55 or Deficient for the Galactosyl-α(1-3) Galactosyl EpitopeJournal of Virology, 78
B. Collins, A. Cotterell, K. McCurry, C. Alvarado, J. Magee, W. Parker, J. Platt (1995)
Cardiac xenografts between primate species provide evidence for the importance of the alpha-galactosyl determinant in hyperacute rejection.Journal of immunology, 154 10
R Larsen, C Rivera-Marrero, L. Ernst, R Cummings, J. Lowe (1990)
Frameshift and nonsense mutations in a human genomic sequence homologous to a murine UDP-Gal:beta-D-Gal(1,4)-D-GlcNAc alpha(1,3)-galactosyltransferase cDNA.The Journal of biological chemistry, 265 12
Sach Sach, Sykes Sykes, Robson Robson, Cooper Cooper (2001)
XenotransplantationAdv Immunol, 79
Russell Other, W. Fodor, J. Springhorn, C. Birks, E. Setter, M. Sandrin, S. Squinto, S. Rollins (1995)
A novel mechanism of retrovirus inactivation in human serum mediated by anti-alpha-galactosyl natural antibodyThe Journal of Experimental Medicine, 182
W. Blanken, D. Eijnden (1985)
Biosynthesis of terminal Gal alpha 1----3Gal beta 1----4GlcNAc-R oligosaccharide sequences on glycoconjugates. Purification and acceptor specificity of a UDP-Gal:N-acetyllactosaminide alpha 1----3-galactosyltransferase from calf thymus.The Journal of biological chemistry, 260 24
M. Sandrin, H. Vaughan, P. Dabkowski, I. McKenzie (1993)
Anti-pig IgM antibodies in human serum react predominantly with Gal(alpha 1-3)Gal epitopes.Proceedings of the National Academy of Sciences of the United States of America, 90 23
R. Hutchison (1981)
How ‘unique’ can meteorites be?Nature, 293
T. Stabzl, T. Marchioro, G. Peters, C. Kiekpatrick, W. Wilson, K. Porter, D. Rifkind, D. Ogden, C. Hitchcock, W. Waddell (1964)
RENAL HETEROTRANSPLANTATION FROM BABOON TO MAN: EXPERIENCE WITH 6 CASESTransplantation, 2
Abstract: Clinical transplantation has become one of the preferred treatments for end‐stage organ failure, and one of the novel approaches being pursued to overcome the limited supply of human organs involves the use of organs from other species. The pig appears to be a near ideal animal due to proximity to humans, domestication, and ability to procreate. The presence of Gal‐α1,3‐Gal residues on the surfaces of pig cells is a major immunological obstacle to xenotransplantation. Alpha1,3galactosyltransferase (α1,3GT) catalyzes the synthesis of Galα1‐3Galβ1‐4GlcNAc‐R (α‐gal epitope) on the glycoproteins and glycolipids of non‐primate mammals, but this does not occur in humans. Moreover, the α‐gal epitope causes hyperacute rejection of pig organs in humans, and thus, the elimination of this antigen from pig tissues is highly desirable. Recently, concerns have been raised that the risk of virus transmission from such pigs may be increased due to the absence of α‐gal on their viral particles. In this study, transgenic cells expressing α1,3GT were selected using 1.25 mg/ml neomycin. The development of HeLa cells expressing α1,3GT now allows accurate studies to be conducted on the function of the α‐gal epitope in xenotransmission. The expressions of α‐gal epitopes on HeLa/α‐gal cells were demonstrated by flow cytometry and confocal microscopy using cells stained with IB4‐fluorescein isothiocyanate lectin. Vaccinia viruses propagated in HeLa/α‐gal cells also expressed α‐gal on their viral envelopes and were more sensitive to inactivation by human sera than vaccinia virus propagated in HeLa cells. Moreover, neutralization of vaccinia virus was inhibited in human serum by 10 mm ethylene glycol bis(β‐aminoethylether)tetraacetic acid (EDTA) treatment. Our data indicated that α‐gal epitopes are one of the major barriers to zoonosis via xenotransmission.
Xenotransplantation – Wiley
Published: Mar 1, 2007
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.