Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Camilloni, A. Rocco, I. Eberini, E. Gianazza, R. Broglia, R. Broglia, G. Tiana (2008)
Urea and guanidinium chloride denature protein L in different ways in molecular dynamics simulations.Biophysical journal, 94 12
S. Khajamohiddin, E. Repalle, A. Pinjari, M. Merrick, D. Siddavattam (2008)
Biodegradation of Aromatic Compounds: An Overview of Meta-Fission Product HydrolasesCritical Reviews in Microbiology, 34
Siliang Gao, Yujun Wang, Xiang-Yang Diao, G. Luo, You-yuan Dai (2010)
Effect of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase in SBA-15.Bioresource technology, 101 11
Ang Li, Y. Qu, Ji-ti Zhou, F. Ma, Hao Zhou, Shengnan Shi (2010)
Characterization of a novel meta-fission product hydrolase from Dyella ginsengisoli LA-4Process Biochemistry, 45
S. Hudson, E. Magner, J. Cooney, B. Hodnett (2005)
Methodology for the immobilization of enzymes onto mesoporous materials.The journal of physical chemistry. B, 109 41
M. Kramer, J. Cruz, P. Pfromm, M. Rezac, P. Czermak (2010)
Enantioselective transesterification by Candida antarctica Lipase B immobilized on fumed silica.Journal of biotechnology, 150 1
Chen Li, M. Hassler, T. Bugg (2008)
Catalytic Promiscuity in the α/β‐Hydrolase Superfamily: Hydroxamic Acid Formation, CC Bond Formation, Ester and Thioester Hydrolysis in the CC Hydrolase FamilyChemBioChem, 9
A. Popat, S. Hartono, F. Stahr, Jian Liu, S. Qiao, Gao Lu (2011)
Mesoporous silica nanoparticles for bioadsorption, enzyme immobilisation, and delivery carriers.Nanoscale, 3 7
Qing-Gui Xiao, Xia Tao, Hai-kui Zou, Jianfeng Chen (2008)
Comparative study of solid silica nanoparticles and hollow silica nanoparticles for the immobilization of lysozymeChemical Engineering Journal, 137
S. Hudson, J. Cooney, E. Magner (2008)
Proteins in mesoporous silicates.Angewandte Chemie, 47 45
M. Holmquist (2000)
Alpha/Beta-hydrolase fold enzymes: structures, functions and mechanisms.Current protein & peptide science, 1 2
Evelyne Weber, D. Sirim, T. Schreiber, Bejoy Thomas, Jurgen Pleiss, M. Hunger, R. Gläser, V. Urlacher (2010)
Immobilization of P450 BM-3 monooxygenase on mesoporous molecular sieves with different pore diametersJournal of Molecular Catalysis B-enzymatic, 64
Pamela Torres‐Salas, Alberto Monte-Martínez, Bessy Cutiño-Ávila, Bárbara Rodriguez-Colinas, M. Alcalde, A. Ballesteros, F. Plou (2011)
Immobilized Biocatalysts: Novel Approaches and Tools for Binding Enzymes to SupportsAdvanced Materials, 23
S. Akgöl, Y. Kaçar, A. Denizli, M. Arica (2001)
Hydrolysis of sucrose by invertase immobilized onto novel magnetic polyvinylalcohol microspheresFood Chemistry, 74
Elina Siirola, Barbara Grischek, Dorina Clay, A. Frank, G. Grogan, W. Kroutil (2011)
Tolerance of β‐diketone hydrolases as representatives of the crotonase superfamily towards organic solventsBiotechnology and Bioengineering, 108
Hui-Ling Ma, Jing He, D. Evans, X. Duan (2004)
Immobilization of lipase in a mesoporous reactor based on MCM-41Journal of Molecular Catalysis B-enzymatic, 30
W. Jin, J. Brennan (2002)
Properties and applications of proteins encapsulated within sol–gel derived materialsAnalytica Chimica Acta, 461
C. Bernal, L. Sierra, M. Mesa (2011)
Application of Hierarchical Porous Silica with a Stable Large Porosity for β‐Galactosidase ImmobilizationChemCatChem, 3
M. Cho, D. Kang, B. Yoon, K. Lee (2000)
Toluene degradation pathway from Pseudomonas putida F1: substrate specificity and gene induction by 1-substituted benzenesJournal of Industrial Microbiology and Biotechnology, 25
H. Ikemoto, Q. Chi, J. Ulstrup (2010)
Stability and Catalytic Kinetics of Horseradish Peroxidase Confined in Nanoporous SBA-15Journal of Physical Chemistry C, 114
Dongyuan Zhao, Jian-yong Feng, Q. Huo, N. Melosh, G. Fredrickson, B. Chmelka, G. Stucky (1998)
Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom poresScience, 279 5350
J. Aburto, M. Ayala, I. Bustos-Jaimes, Carmina Montiel, E. Terrés, J. Domínguez, E. Torres (2005)
Stability and catalytic properties of chloroperoxidase immobilized on SBA-16 mesoporous materialsMicroporous and Mesoporous Materials, 83
G. Yadav, Sachin Jadhav (2005)
Synthesis of reusable lipases by immobilization on hexagonal mesoporous silica and encapsulation in calcium alginate : Transesterification in non-aqueous mediumMicroporous and Mesoporous Materials, 86
Sarah Hudson, Jakki Cooney, Edmond Magner (2008)
Proteine in mesoporösen SilicatenAngewandte Chemie, 120
S. Hudson, J. Cooney, and Hodnett, E. Magner (2007)
Chloroperoxidase on Periodic Mesoporous Organosilanes: Immobilization and ReuseChemistry of Materials, 19
A. and, X. Zhao (2003)
Functionalization of SBA-15 with APTES and Characterization of Functionalized MaterialsJournal of Physical Chemistry B, 107
J. Gómez, M. Romero, T. Fernandez, Saúl García (2010)
Immobilization and enzymatic activity of β-glucosidase on mesoporous SBA-15 silicaJournal of Porous Materials, 17
H. Essa, E. Magner, J. Cooney, B. Hodnett (2007)
Influence of pH and ionic strength on the adsorption, leaching and activity of myoglobin immobilized onto ordered mesoporous silicatesJournal of Molecular Catalysis B-enzymatic, 49
A molecular simulation strategy based on homology modeling and electrostatic potential calculations has been proposed to assist the immobilization process of a CC hydrolase MfphA onto mesoporous SBA‐15. The size of the enzyme, pH‐dependence of the immobilization process, and possible orientation of MfphA onto mesoporous materials (MPs) were predicted by molecular simulation. The adsorption of MfphA onto SBA‐15 reached equilibrium in 1.5 hours at pH 7.0 and the maximum loading capacity was 34 mg g−1. Meanwhile at pH 9.0, no obvious adsorption was observed after 12 hours which corresponded to the molecular simulation prediction. It was also shown that, after immobilization, the catalytic activity of immobilized MfphA decreased to some extent, but the thermal stability was significantly improved. Guanidinium chloride (GdmCl) exhibited similar activity attenuation for both immobilized and free enzyme. By contrast, the immobilized MfphA was more resistant to urea at high concentration than was the free enzyme. Meanwhile, recycling experiments showed that the immobilized enzyme retained 30 % of its initial activity after ten reaction cycles.
ChemPlusChem – Wiley
Published: Apr 1, 2012
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.