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Methylation, crystallization and SAD phasing of the Csu pilus CsuC–CsuE chaperone–adhesin subunit pre‐assembly complex from Acinetobacter baumannii

Methylation, crystallization and SAD phasing of the Csu pilus CsuC–CsuE chaperone–adhesin subunit... Acinetobacter baumannii is one of the most difficult Gram‐negative bacteria to control and treat. This pathogen forms biofilms on hospital surfaces and medical devices using Csu pili assembled via the archaic chaperone–usher pathway. To uncover the mechanism of bacterial attachment to abiotic surfaces, it was aimed to determine the crystal structure of the pilus tip adhesin CsuE. The CsuC–CsuE chaperone–subunit pre‐assembly complex was purified from the periplasm of Escherichia coli overexpressing CsuC and CsuE. Despite the high purity of the complex, no crystals could be obtained. This challenge was solved by the methylation of lysine residues. The complex was crystallized in 0.1 M bis‐tris pH 5.5, 17% PEG 3350 using the hanging‐drop vapour‐diffusion method. The crystals diffracted to a resolution of 2.31 Å and belonged to the triclinic space group P1, with unit‐cell parameters a = 53.84, b = 63.85, c = 89.25 Å, α = 74.65, β = 79.65, γ = 69.07°. Initial phases were derived from a single anomalous diffraction experiment using a selenomethionine derivative. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Methylation, crystallization and SAD phasing of the Csu pilus CsuC–CsuE chaperone–adhesin subunit pre‐assembly complex from Acinetobacter baumannii

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Publisher
Wiley
Copyright
Copyright © 2017 Wiley Subscription Services
ISSN
2053-230X
eISSN
2053-230X
DOI
10.1107/S2053230X17009566
pmid
28777087
Publisher site
See Article on Publisher Site

Abstract

Acinetobacter baumannii is one of the most difficult Gram‐negative bacteria to control and treat. This pathogen forms biofilms on hospital surfaces and medical devices using Csu pili assembled via the archaic chaperone–usher pathway. To uncover the mechanism of bacterial attachment to abiotic surfaces, it was aimed to determine the crystal structure of the pilus tip adhesin CsuE. The CsuC–CsuE chaperone–subunit pre‐assembly complex was purified from the periplasm of Escherichia coli overexpressing CsuC and CsuE. Despite the high purity of the complex, no crystals could be obtained. This challenge was solved by the methylation of lysine residues. The complex was crystallized in 0.1 M bis‐tris pH 5.5, 17% PEG 3350 using the hanging‐drop vapour‐diffusion method. The crystals diffracted to a resolution of 2.31 Å and belonged to the triclinic space group P1, with unit‐cell parameters a = 53.84, b = 63.85, c = 89.25 Å, α = 74.65, β = 79.65, γ = 69.07°. Initial phases were derived from a single anomalous diffraction experiment using a selenomethionine derivative.

Journal

Acta Crystallographica Section FWiley

Published: Jan 1, 2017

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