Access the full text.
Sign up today, get DeepDyve free for 14 days.
G. Kempermann, D. Gast, G. Kronenberg, M. Yamaguchi, F. Gage (2003)
Early determination and long-term persistence of adult-generated new neurons in the hippocampus of mice, 130
C. Lavebratt, Alexandra Trifunovski, Ann-Sophie Persson, Fu‐Hua Wang, T. Klason, Inger Öhman, A. Josephsson, L. Olson, C. Spenger, M. Schalling (2006)
Carbamazepine protects against megencephaly and abnormal expression of BDNF and Nogo signaling components in the mceph/mceph mouseNeurobiology of Disease, 24
H. Misonou, J. Trimmer (2004)
Determinants of Voltage-Gated Potassium Channel Surface Expression and Localization in Mammalian NeuronsCritical Reviews in Biochemistry and Molecular Biology, 39
T. Palmer, Andrew Willhoite, F. Gage (2000)
Vascular niche for adult hippocampal neurogenesisJournal of Comparative Neurology, 425
H. Cameron, C. Woolley, B. McEwen, E. Gould (1993)
Differentiation of newly born neurons and glia in the dentate gyrus of the adult ratNeuroscience, 56
S. Petersson, Ann-Sophie Persson, J. Johansen, M. Ingvar, J. Nilsson, G. Klement, P. Århem, M. Schalling, C. Lavebratt (2003)
Truncation of the Shaker‐like voltage‐gated potassium channel, Kv1.1, causes megencephalyEuropean Journal of Neuroscience, 18
D. Abrous, M. Koehl, M. Moal (2005)
Adult neurogenesis: from precursors to network and physiology.Physiological reviews, 85 2
S. Jessberger, B. Römer, H. Babu, G. Kempermann (2005)
Seizures induce proliferation and dispersion of doublecortin-positive hippocampal progenitor cellsExperimental Neurology, 196
M. West (1993)
Regionally specific loss of neurons in the aging human hippocampusNeurobiology of Aging, 14
H. Gundersen, P. Bagger, T. Bendtsen, S. Evans, L. Korbo, N. Marcussen, A. Møller, K. Nielsen, J. Nyengaard, B. Pakkenberg, Sørensen Fb, A. Vesterby, M. West (1988)
The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosisAPMIS, 96
Angela Lee, T. Dumas, Phiroz Tarapore, Brian Webster, D. Ho, D. Kaufer, R. Sapolsky (2003)
Potassium channel gene therapy can prevent neuron death resulting from necrotic and apoptotic insultsJournal of Neurochemistry, 86
D. Ekhterae, O. Platoshyn, S. Krick, Ying Yu, S. McDaniel, J. Yuan (2001)
Bcl-2 decreases voltage-gated K+ channel activity and enhances survival in vascular smooth muscle cells.American journal of physiology. Cell physiology, 281 1
J. Parent, S. Janumpalli, J. McNamara, D. Lowenstein (1998)
Increased dentate granule cell neurogenesis following amygdala kindling in the adult ratNeuroscience Letters, 247
J. Hinds (1968)
Autoradiographic study of histogenesis in the mouse olfactory bulb I. Time of origin of neurons and neurogliaJournal of Comparative Neurology, 134
J. Parent, T. Yu, Rebecca Leibowitz, D. Geschwind, R. Sloviter, D. Lowenstein (1997)
Dentate Granule Cell Neurogenesis Is Increased by Seizures and Contributes to Aberrant Network Reorganization in the Adult Rat HippocampusThe Journal of Neuroscience, 17
H. Gundersen (1988)
The nucleatorJournal of Microscopy, 151
D. Lie, Hongjun Song, S. Colamarino, G. Ming, Fred Gage (2004)
Neurogenesis in the adult brain: new strategies for central nervous system diseases.Annual review of pharmacology and toxicology, 44
F. Sun, Xin Guo (2005)
Molecular and cellular mechanisms of neuroprotection by vascular endothelial growth factorJournal of Neuroscience Research, 79
Junying Yuan, B. Yankner (2000)
Apoptosis in the nervous systemNature, 407
Ann-Sophie Persson, G. Klement, Malin Almgren, K. Sahlholm, J. Nilsson, S. Petersson, P. Århem, M. Schalling, C. Lavebratt (2005)
A truncated Kv1.1 protein in the brain of the megencephaly mouse: expression and interactionBMC Neuroscience, 6
S. Petersson, A. Nordqvist, M. Schalling, C. Lavebratt (1999)
The megencephaly mouse has disturbances in the insulin-like growth factor (IGF) system.Brain research. Molecular brain research, 72 1
Jaewon Lee, W. Duan, M. Mattson (2002)
Evidence that brain‐derived neurotrophic factor is required for basal neurogenesis and mediates, in part, the enhancement of neurogenesis by dietary restriction in the hippocampus of adult miceJournal of Neurochemistry, 82
J. Hallows, B. Tempel (1998)
Expression of Kv1.1, a Shaker-Like Potassium Channel, Is Temporally Regulated in Embryonic Neurons and GliaThe Journal of Neuroscience, 18
S. Petersson, C. Lavebratt, M. Schalling, T. Hökfelt (2000)
Expression of cholecystokinin, enkephalin, galanin and neuropeptide Y is markedly changed in the brain of the megencephaly mouseNeuroscience, 100
J. Parent, D. Lowenstein (2002)
Seizure-induced neurogenesis: are more new neurons good for an adult brain?Progress in brain research, 135
J. Altman, G. Das (1965)
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in ratsJournal of Comparative Neurology, 124
W. Humphrey, Hongxin Dong, C. Csernansky, J. Csernansky (2002)
Immediate and delayed hippocampal neuronal loss induced by kainic acid during early postnatal development in the rat.Brain research. Developmental brain research, 137 1
H. Gundersen, E. Jensen (1987)
The efficiency of systematic sampling in stereology and its prediction *Journal of Microscopy, 147
M. Diez, P. Schweinhardt, S. Petersson, Fu‐Hua Wang, C. Lavebratt, M. Schalling, T. Hökfelt, C. Spenger (2003)
MRI and in situ hybridization reveal early disturbances in brain size and gene expression in the megencephalic (mceph/mceph) mouseEuropean Journal of Neuroscience, 18
H. Ouadid‐Ahidouch, F. Chaussade, M. Roudbaraki, C. Slomianny, E. Dewailly, P. Delcourt, N. Prevarskaya (2000)
KV1.1 K(+) channels identification in human breast carcinoma cells: involvement in cell proliferation.Biochemical and biophysical research communications, 278 2
Kaplan, Jw Hinds (1977)
Neurogenesis in the adult rat: electron microscopic analysis of light radioautographs.Science, 197 4308
L. Donahue, S. Cook, K. Johnson, R. Bronson, M. Davissen (1996)
Megencephaly: a new mouse mutation on chromosome 6 that causes hypertrophy of the brainMammalian Genome, 7
J. Ruppersberg (1996)
Ion Channels in Excitable Membranes
C. Lois, A. Álvarez-Buylla (1993)
Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia.Proceedings of the National Academy of Sciences of the United States of America, 90
K. Dorph‐Petersen, J. Nyengaard, H. Gundersen (2001)
Tissue shrinkage and unbiased stereological estimation of particle number and size *Journal of Microscopy, 204
The megencephaly mice show dramatic progressive increase in brain size and seizures. The overgrowth affects primarily the hippocampus and ventral cortex. The phenotype originates from a mutation in the Shaker‐like voltage‐gated potassium channel Kv1.1 brain, which results in a malfunctioning protein. A key question in elucidating the mechanism behind the unique brain overgrowth is whether it is caused by an increase in cell number. By applying stereological techniques, we found that the number of both neurons and astrocytes, as well as structure volume, was increased approximately two‐fold within dentate gyrus (DG), CA2/3, and hilus of 12‐week‐old mceph/mceph versus wild type mice. In CA1, there was a tendency toward an increase in volume and in number of astrocytes. The volume estimates in newborn and p14 mice suggest that the overgrowth in mceph/mceph hippocampus starts between birth and the second week of life. To investigate the hyperplasia, cell proliferation was studied within the subgranular zone of the DG using BrdU and Ki67. There was a three‐fold increase in proliferation in mceph/mceph mice compared to wild type mice at an age before onset of epileptic symptoms (3 weeks), and these new mceph/mceph neurons showed increased migration and had a 6‐week survival rate as the new neurons in wild type DG. Also when seizures were frequent in mceph/mceph (9 weeks old), the proliferation rate was three‐fold higher than in wild type. The number of TUNEL‐positive cells in hippocampus was lower in mceph/mceph supporting additional overgrowth mechanism than induced by seizures. In conclusion, lack of a functional Kv1.1 ion channel subunit in the mceph/mceph mice causes a unique neuronal hyperplasia in distinct hippocampal regions and consequently hippocampal enlargement from 2 to 3 weeks of age. This phenotype is a result, at least in DG, from increased proliferation, neurogenesis, and enhanced general hippocampal cell survival. © 2007 Wiley‐Liss, Inc.
Hippocampus – Wiley
Published: Jan 1, 2007
Keywords: ; ; ; ;
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.