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Lack of antibody production against Hanganutziu‐Deicher (H‐D) antigens with N‐glycolylneuraminic acid in patients with porcine exposure history

Lack of antibody production against Hanganutziu‐Deicher (H‐D) antigens with N‐glycolylneuraminic... Abstract: The significance of non‐αgalactosyl antigens remains unclear in pig‐to‐primate xenotransplantation. Hanganutziu‐Deicher (H‐D) antigens with terminal N‐glycolylneuraminic acid (NeuGc) are widely expressed on endothelial cells of mammalian species, with the exception of humans. As baboons and monkeys also express H‐D antigens, a pig‐to‐non‐human primate experimental model cannot resolve the question of whether H‐D antigens can elicit a potent humoral response in human recipients. The purpose of this study was to elucidate the clinical significance of H‐D antigens by examining the sera from patients who have been previously exposed to porcine tissue. After the digestion of porcine aortic endothelial cells (PAEC) by neuraminidase, NeuGc and N‐acetylneuraminic acid (NeuAc) were quantitated by HPLC. IgG and IgM antibody levels against H‐D antigens were measured by NeuGc‐GM3‐coated ELISA plates in the sera of patients who had undergone ex vivo kidney perfusion 1 to 3 weeks and 2 years previously (n = 2) or had been injected with fetal porcine islets 2 months previously (n = 10). HPLC determined that 9.7×107 NeuAc and 6.3×107 NeuGc residues per cell were released from PAEC by neuraminidase, while 25.7×107 NeuAc and an undetectable level of NeuGc were released from human aortic endothelial cells (HAEC). No significant elevation of IgG or IgM antibody levels against NeuGc‐GM3 was observed in sera from patients with a history of porcine exposure. Considering the active production of antibody against the foreign galactosyl antigens after pig‐to‐human xenotransplantation, some production of antibodies against the equally foreign H‐D antigens would be expected, because large amounts of NeuGc terminated saccharides are present in the pig endothelial cell surface. However, no production of antibodies directed to H‐D antigens could be found in patients exposed to porcine tissue. Further studies are warranted to explain why H‐D antigens do not elicit a significant antibody production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Lack of antibody production against Hanganutziu‐Deicher (H‐D) antigens with N‐glycolylneuraminic acid in patients with porcine exposure history

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References (23)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1034/j.1399-3089.2000.00056.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: The significance of non‐αgalactosyl antigens remains unclear in pig‐to‐primate xenotransplantation. Hanganutziu‐Deicher (H‐D) antigens with terminal N‐glycolylneuraminic acid (NeuGc) are widely expressed on endothelial cells of mammalian species, with the exception of humans. As baboons and monkeys also express H‐D antigens, a pig‐to‐non‐human primate experimental model cannot resolve the question of whether H‐D antigens can elicit a potent humoral response in human recipients. The purpose of this study was to elucidate the clinical significance of H‐D antigens by examining the sera from patients who have been previously exposed to porcine tissue. After the digestion of porcine aortic endothelial cells (PAEC) by neuraminidase, NeuGc and N‐acetylneuraminic acid (NeuAc) were quantitated by HPLC. IgG and IgM antibody levels against H‐D antigens were measured by NeuGc‐GM3‐coated ELISA plates in the sera of patients who had undergone ex vivo kidney perfusion 1 to 3 weeks and 2 years previously (n = 2) or had been injected with fetal porcine islets 2 months previously (n = 10). HPLC determined that 9.7×107 NeuAc and 6.3×107 NeuGc residues per cell were released from PAEC by neuraminidase, while 25.7×107 NeuAc and an undetectable level of NeuGc were released from human aortic endothelial cells (HAEC). No significant elevation of IgG or IgM antibody levels against NeuGc‐GM3 was observed in sera from patients with a history of porcine exposure. Considering the active production of antibody against the foreign galactosyl antigens after pig‐to‐human xenotransplantation, some production of antibodies against the equally foreign H‐D antigens would be expected, because large amounts of NeuGc terminated saccharides are present in the pig endothelial cell surface. However, no production of antibodies directed to H‐D antigens could be found in patients exposed to porcine tissue. Further studies are warranted to explain why H‐D antigens do not elicit a significant antibody production.

Journal

Xenotransplantation – Wiley

Published: Aug 1, 2000

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