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M. Rizzari, T. Suszynski, L. Kidder, S. Stein, T. O’Brien, V. Sajja, W. Scott, V. Kirchner, B. Weegman, E. Avgoustiniatos, P. Todd, D. Kennedy, B. Hammer, D. Sutherland, B. Hering, K. Papas (2010)
Surgical protocol involving the infusion of paramagnetic microparticles for preferential incorporation within porcine islets.Transplantation proceedings, 42 10
D. Windt, D. Windt, R. Bottino, Anna Casu, N. Campanile, C. Smetanka, Jing He, N. Murase, H. Hara, S. Ball, B. Loveland, D. Ayares, F. Lakkis, D. Cooper, M. Trucco (2009)
Long‐Term Controlled Normoglycemia in Diabetic Non‐Human Primates After Transplantation with hCD46 Transgenic Porcine IsletsAmerican Journal of Transplantation, 9
Ferrer (2008)
Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolationTransplantation, 86
P. Thompson, K. Cardona, M. Russell, I. Badell, V. Shaffer, G. Korbutt, G. Rayat, J. Cano, Mingqing Song, W. Jiang, E. Strobert, Rajotte Rv, T. Pearson, Allan Kirk, C. Larsen (2011)
CD40‐Specific Costimulation Blockade Enhances Neonatal Porcine Islet Survival in Nonhuman PrimatesAmerican Journal of Transplantation, 11
D. Dufrane, W. D'Hoore, R. Goebbels, A. Saliez, Y. Guiot, P. Gianello (2006)
Parameters favouring successful adult pig islet isolations for xenotransplantation in pig‐to‐primate modelsXenotransplantation, 13
Jae Kim, Hyoung-il Kim, Kang‐Woo Lee, J. Yu, Sun Kim, Ho Park, Chung-Gyu Park, S. Ihm, J. Ha, S. Kim, Hong-Kyu Lee, Curie Ahn, Kyong Park (2007)
Influence of strain and age differences on the yields of porcine islet isolation: extremely high islet yields from SPF CMS miniature pigsXenotransplantation, 14
B. Hering, M. Wijkstrom, M. Graham, M. Hårdstedt, T. Aasheim, T. Jie, J. Ansite, M. Nakano, Jane Cheng, Wei Li, K. Moran, U. Christians, Colleen Finnegan, C. Mills, D. Sutherland, P. Bansal‐Pakala, M. Murtaugh, N. Kirchhof, H. Schuurman (2006)
Prolonged diabetes reversal after intraportal xenotransplantation of wild-type porcine islets in immunosuppressed nonhuman primatesNature Medicine, 12
D. Lourenço-, R. Toledo, F. Coutinho, M. Cavalcanti, J. Correia‐Deur, F. Montenegro, S. Siqueira, L. Margarido, M. Machado, S. Toledo (2008)
INHIBITION OF CASEIN KINASE II: A POTENTIAL THERAPEUTIC STRATEGY FOR PANCREATIC CANCERPancreas, 37
Hyoung-il Kim, Song-Yi Lee, Sang-Man Jin, K. Kim, J. Yu, S. Yeom, T. Yoon, Jae Kim, J. Ha, Chung-Gyu Park, Sang‐Joon Kim (2009)
Parameters for successful pig islet isolation as determined using 68 specific‐pathogen‐free miniature pigsXenotransplantation, 16
Jiang X, Qian T, Linn T, Cao L, Xiang G, Wang Y, Peng H, Xue P, Zhang L, Chen D, Yang X. Islet isolation and purification from inbred Wuzhishan miniature pigs. Xenotransplantation 2012; 19: 159–165. © 2012 John Wiley & Sons A/S. Abstract: Background: To investigate the applicability of inbred Wuzhishan (WZS) miniature pigs for porcine islet isolation and purification. Methods: Islet isolation and purification was conducted on adult (1‐yr‐old), male inbred WZS miniature pigs and age‐ and sex‐matched market pigs obtained from a local slaughterhouse (control group). Pancreata were excised, and islet isolation was carried out by static digestion and discontinuous gradient centrifugation. Viability of the purified islets was tested by radioimmunochemistry assay to measure glucose‐induced insulin release in culture and transplantation in an in vivo study. Results: The anatomical structure of the WZS miniature pig pancreas was more similar to the human pancreas than that of the market pig. Islet yield of the WZS miniature pigs’ pancreata was significantly higher than that of the market pigs (6078 ± 1105 vs. 2500 ± 625 islet equivalents (IEQ)/g). In vitro study demonstrated that the islets isolated from WZS miniature pigs were viable, as they efficiently responded to glucose challenge. In vivo study showed that the islets from both groups could cure the diabetic rat with the survival varied from 3 to 5 days (median, 4.3 days) and 2–4 days (median, 3.6 days) in experimental group and control group, respectively. Conclusion: Wuzhishan miniature pig pancreas may be a feasible source of islets for xenotransplantation.
Xenotransplantation – Wiley
Published: May 1, 2012
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