Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Interleukin‐15 enhances CD4 + CD45RA + expression on umbilical cord blood mononuclear cells

Interleukin‐15 enhances CD4 + CD45RA + expression on umbilical cord blood mononuclear cells The reduced incidence of graft‐vs.‐host disease following umbilical cord blood (CB) transplantation may be related to the functional immaturity of newborn T cells expressing mainly the naive CD45RA phenotype. Expansion of CD4+ CD45RA+ T cells using cytokines may benefit neonates and infants with human immunodeficiency virus (HIV) infection, as a preferential decline in CD4+ CD45RA+ cells has been noted as HIV disease progresses. The aim of the study was to investigate the effect of interleukin (IL)‐15, a novel cytokine similar to IL‐2 in biological activities, on CD45RA/RO expression and apoptosis in umbilical cord blood (CB) and adult peripheral blood (APB) mononuclear cells (MNCs). Prior to culture, CB MNCs contained a greater number of CD4+ CD45RA+ cells and fewer CD4+ CD45RO+ cells than did APB MNCs. When incubated with RPMI‐1640 containing 10% fetal calf serum for 7 days, the percentage of CD45RA+ cells within CD4+ T cells (%CD45RA+/CD4+) significantly decreased compared to that of fresh CB MNCs. IL‐15 exerted a dose‐dependent increase of %CD45RA+/CD4+ and a corresponding decrease of %CD45RO+/CD4+ in CB MNCs, an effect not observed with APB MNCs treated with IL‐15. The percentages of CD45RA+ and CD45RO+ expression within CD8+ cells, however, were not influenced by IL‐15, in either CB or APB MNCs. A greater number of CB MNCs underwent apoptosis than did APB MNCs after 7 days of culture in RPMI‐1640 containing 10% fetal calf serum. IL‐15 did not inhibit apoptosis but induced proliferation comparable to that achieved in APB MNCs. The ability of IL‐15 to preferentially enhance the proliferation of CD4+ CD45RA+ cells in CB MNCs suggests a role for immunomodulative therapy in HIV‐infected newborns and infants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pediatric Allergy and Immunology Wiley

Interleukin‐15 enhances CD4 + CD45RA + expression on umbilical cord blood mononuclear cells

Loading next page...
 
/lp/wiley/interleukin-15-enhances-cd4-cd45ra-expression-on-umbilical-cord-blood-RNHKyj2Erc

References (29)

Publisher
Wiley
Copyright
Copyright © 2001 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0905-6157
eISSN
1399-3038
DOI
10.1034/j.1399-3038.2001.012004188.x
Publisher site
See Article on Publisher Site

Abstract

The reduced incidence of graft‐vs.‐host disease following umbilical cord blood (CB) transplantation may be related to the functional immaturity of newborn T cells expressing mainly the naive CD45RA phenotype. Expansion of CD4+ CD45RA+ T cells using cytokines may benefit neonates and infants with human immunodeficiency virus (HIV) infection, as a preferential decline in CD4+ CD45RA+ cells has been noted as HIV disease progresses. The aim of the study was to investigate the effect of interleukin (IL)‐15, a novel cytokine similar to IL‐2 in biological activities, on CD45RA/RO expression and apoptosis in umbilical cord blood (CB) and adult peripheral blood (APB) mononuclear cells (MNCs). Prior to culture, CB MNCs contained a greater number of CD4+ CD45RA+ cells and fewer CD4+ CD45RO+ cells than did APB MNCs. When incubated with RPMI‐1640 containing 10% fetal calf serum for 7 days, the percentage of CD45RA+ cells within CD4+ T cells (%CD45RA+/CD4+) significantly decreased compared to that of fresh CB MNCs. IL‐15 exerted a dose‐dependent increase of %CD45RA+/CD4+ and a corresponding decrease of %CD45RO+/CD4+ in CB MNCs, an effect not observed with APB MNCs treated with IL‐15. The percentages of CD45RA+ and CD45RO+ expression within CD8+ cells, however, were not influenced by IL‐15, in either CB or APB MNCs. A greater number of CB MNCs underwent apoptosis than did APB MNCs after 7 days of culture in RPMI‐1640 containing 10% fetal calf serum. IL‐15 did not inhibit apoptosis but induced proliferation comparable to that achieved in APB MNCs. The ability of IL‐15 to preferentially enhance the proliferation of CD4+ CD45RA+ cells in CB MNCs suggests a role for immunomodulative therapy in HIV‐infected newborns and infants.

Journal

Pediatric Allergy and ImmunologyWiley

Published: Aug 1, 2001

There are no references for this article.