Access the full text.
Sign up today, get DeepDyve free for 14 days.
(2005)
Microbial Cell Factories BioMed Central Review
Jun Li, Can Attila, Liang Wang, T. Wood, J. Valdés, W. Bentley (2007)
Quorum Sensing in Escherichia coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm ArchitectureJournal of Bacteriology, 189
W. Fuqua, S. Winans, E. Greenberg (1994)
Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulatorsJournal of Bacteriology, 176
Varnika Roy, Jacqueline Smith, Jingxin Wang, Jessica Stewart, W. Bentley, H. Sintim (2010)
Synthetic analogs tailor native AI-2 signaling across bacterial species.Journal of the American Chemical Society, 132 32
Sonja Gamby, Varnika Roy, Min Guo, Jacqueline Smith, Jingxin Wang, Jessica Stewart, Xiao Wang, W. Bentley, H. Sintim (2012)
Altering the communication networks of multispecies microbial systems using a diverse toolbox of AI-2 analogues.ACS chemical biology, 7 6
Christian Schlieker, B. Bukau, A. Mogk (2002)
Prevention and reversion of protein aggregation by molecular chaperones in the E. coli cytosol: implications for their applicability in biotechnology.Journal of biotechnology, 96 1
E. Gospodarek, Tomasz Bogiel, P. Zalas-Więcek (2009)
Communication between microorganisms as a basis for production of virulence factors.Polish journal of microbiology, 58 3
L. Keller, M. Surette (2006)
Communication in bacteria: an ecological and evolutionary perspectiveNature Reviews Microbiology, 4
D. Bolen, I. Baskakov (2001)
The osmophobic effect: natural selection of a thermodynamic force in protein folding.Journal of molecular biology, 310 5
J. Ha, Y. Eo, A. Grishaev, Min Guo, Jacqueline Smith, H. Sintim, Eun-hee Kim, H. Cheong, W. Bentley, K. Ryu (2013)
Crystal structures of the LsrR proteins complexed with phospho-AI-2 and two signal-interrupting analogues reveal distinct mechanisms for ligand recognition.Journal of the American Chemical Society, 135 41
N. Oganesyan, Irina Ankoudinova, Sung-Hou Kim, R. Kim (2007)
Effect of osmotic stress and heat shock in recombinant protein overexpression and crystallization.Protein expression and purification, 52 2
Jingqiu Chen, T. Acton, S. Basu, G. Montelione, M. Inouye (2002)
Enhancement of the solubility of proteins overexpressed in Escherichia coli by heat shock.Journal of molecular microbiology and biotechnology, 4 6
Jeffrey Thomas, F. Baneyx (1996)
Protein Misfolding and Inclusion Body Formation in Recombinant Escherichia coli Cells Overexpressing Heat-shock Proteins (*)The Journal of Biological Chemistry, 271
(2002)
Increasing the soluble expression and crystallization of LsrK Acta Cryst
Varnika Roy, R. Fernandes, C. Tsao, W. Bentley (2010)
Cross species quorum quenching using a native AI-2 processing enzyme.ACS chemical biology, 5 2
LsrK is one of the key components of the luxS‐regulated (lsr) operon in Escherichia coli and plays an important role during the quorum‐sensing (QS) process mediated by autoinducer‐2 (AI‐2). The AI‐2 molecule is imported into the cell by the LsrACB transporter and is subsequently phosphorylated (to AI‐2‐P) by LsrK. AI‐2‐P binds to the repressor protein of the lsr operon (LsrR) and triggers various cellular responses related to QS by dissociating LsrR from the DNA. Although a large amount of purified LsrK is required for structural studies, recombinant GST‐LsrK was mostly expressed in an insoluble form. To enhance the soluble expression of LsrK, an attempt was made to increase the expression of the cellular chaperone proteins that are well known to support proper protein folding. Transformed E. coli was cultured in high‐salt LB medium and heat shock was applied prior to subsequent IPTG induction at 20°C. These procedures increased the yield of purified LsrK by about tenfold compared with standard IPTG induction at 20°C. The expressed LsrK was readily purified by GST‐affinity chromatography. Crystals of LsrK were grown by the hanging‐drop vapour‐diffusion method. The X‐ray diffraction data of the crystal were processed in a primitive hexagonal space group to 2.9 Å resolution.
Acta Crystallographica Section F – Wiley
Published: Jan 1, 2017
Keywords: ; ; ; ; ; ;
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.