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Increased respiratory burst response and alkaline phosphatase activity in human cord blood neutrophils

Increased respiratory burst response and alkaline phosphatase activity in human cord blood... The priming effect of a bacterial lipopolysaccharide (LPS) on subsequent respiratory burst activity induced by either the chemoattractant formylmethionyl‐leucyi‐phenylalaninc (fMLP) or phorbol myristate acetate (PMA; a protein kinase C activator) was studied in human ncutrophils isolated from cord blood and adult peripheral blood. Cells from adults, but not from newborn babies, were primed by LPS pretreatment. The content and release of β‐glucuronidase and vitamin‐B12 binding protein, or marker for the azurophilic and specific granules, respectively, was similar for cells from infants and adult controls. In contrast, alkaline phosphatase activity was significantly increased in the cord blood neutrophils compared to neutrophils from adult peripheral blood. The latency of the alkaline phosphatase activity was, however, similar. Thus, the primed response of cord blood neutrophils could not be explained by an increased release of azurophilic or specific granule content. If the increased alkaline phosphatase activity of curd cells represents an increased number of secretory vesicles, however, then this would indicate a rapid turnover leading to delivery of new receptors and oxidase components to the cell membrane. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pediatric Allergy and Immunology Wiley

Increased respiratory burst response and alkaline phosphatase activity in human cord blood neutrophils

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References (37)

Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0905-6157
eISSN
1399-3038
DOI
10.1111/j.1399-3038.1992.tb00032.x
Publisher site
See Article on Publisher Site

Abstract

The priming effect of a bacterial lipopolysaccharide (LPS) on subsequent respiratory burst activity induced by either the chemoattractant formylmethionyl‐leucyi‐phenylalaninc (fMLP) or phorbol myristate acetate (PMA; a protein kinase C activator) was studied in human ncutrophils isolated from cord blood and adult peripheral blood. Cells from adults, but not from newborn babies, were primed by LPS pretreatment. The content and release of β‐glucuronidase and vitamin‐B12 binding protein, or marker for the azurophilic and specific granules, respectively, was similar for cells from infants and adult controls. In contrast, alkaline phosphatase activity was significantly increased in the cord blood neutrophils compared to neutrophils from adult peripheral blood. The latency of the alkaline phosphatase activity was, however, similar. Thus, the primed response of cord blood neutrophils could not be explained by an increased release of azurophilic or specific granule content. If the increased alkaline phosphatase activity of curd cells represents an increased number of secretory vesicles, however, then this would indicate a rapid turnover leading to delivery of new receptors and oxidase components to the cell membrane.

Journal

Pediatric Allergy and Immunology – Wiley

Published: May 1, 1992

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