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Inactivation of porcine endogenous retrovirus in pigs using CRISPR‐Cas9, editorial commentary

Inactivation of porcine endogenous retrovirus in pigs using CRISPR‐Cas9, editorial commentary In a recent issue of Science, the group of George Church at Harvard Medical School and Luhan Yang at the Boston‐based company eGenesis, in collaboration with groups in China, published the live birth of pigs in which the genes for PERV were inactivated using the CRISPR‐Cas9 gene‐editing technology. This paper is a logical follow‐up of their earlier publication in Science, 2015, in which the successful inactivation of PERV genes in the porcine PK15 cell line was documented. Joachim Denner has written a commentary about this first publication in the columns of Xenotransplantation. The present publication has attracted considerable attention, and we herewith write our commentary for the readership of Xenotransplantation.The major achievement in the recent Science paper is the generation of live piglets in which PERV genes were inactivated using the CRISPR‐Cas9 technology. The authors started with primary cells, porcine fetal fibroblast cells called FFF3. The first achievement was the repeat of the success in the immortalized PK15 cell line, that is, PERV inactivation in these primary cells using the CRISPR‐Cas9 technology. This required modifications in the experimental approach to increase the target efficiency of which the long‐term implications are yet to be determined. Twenty‐five copies of the pol http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Inactivation of porcine endogenous retrovirus in pigs using CRISPR‐Cas9, editorial commentary

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References (25)

Publisher
Wiley
Copyright
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/xen.12363
pmid
29131463
Publisher site
See Article on Publisher Site

Abstract

In a recent issue of Science, the group of George Church at Harvard Medical School and Luhan Yang at the Boston‐based company eGenesis, in collaboration with groups in China, published the live birth of pigs in which the genes for PERV were inactivated using the CRISPR‐Cas9 gene‐editing technology. This paper is a logical follow‐up of their earlier publication in Science, 2015, in which the successful inactivation of PERV genes in the porcine PK15 cell line was documented. Joachim Denner has written a commentary about this first publication in the columns of Xenotransplantation. The present publication has attracted considerable attention, and we herewith write our commentary for the readership of Xenotransplantation.The major achievement in the recent Science paper is the generation of live piglets in which PERV genes were inactivated using the CRISPR‐Cas9 technology. The authors started with primary cells, porcine fetal fibroblast cells called FFF3. The first achievement was the repeat of the success in the immortalized PK15 cell line, that is, PERV inactivation in these primary cells using the CRISPR‐Cas9 technology. This required modifications in the experimental approach to increase the target efficiency of which the long‐term implications are yet to be determined. Twenty‐five copies of the pol

Journal

Xenotransplantation – Wiley

Published: Jan 1, 2017

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