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In‐house SIRAS phasing of the polyunsaturated fatty‐acid isomerase from Propionibacterium acnes

In‐house SIRAS phasing of the polyunsaturated fatty‐acid isomerase from Propionibacterium acnes The polyenoic fatty‐acid isomerase from Propionibacterium acnes (PAI) catalyzes the double‐bond isomerization of linoleic acid to conjugated linoleic acid, which is a dairy‐ or meat‐derived fatty acid in the human diet. PAI was overproduced in Escherichia coli and purified to homogeneity as a yellow‐coloured protein. The nature of the bound cofactor was analyzed by absorption and fluorescence spectroscopy. Single crystals of PAI were obtained in two crystal forms. Cubic shaped crystals belong to space group I213, with a unit‐cell parameter of 160.4 Å, and plate‐like crystals belong to the monoclinic space group C2, with unit‐cell parameters a = 133.7, b = 60.8, c = 72.2 Å, β = 115.8°. Both crystal forms contain one molecule per asymmetric unit and diffract to a resolution of better than 2.0 Å. Initial phases were obtained by SIRAS from in‐house data from a cubic crystal that was soaked with an unusually low KI concentration of 0.25 M. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

In‐house SIRAS phasing of the polyunsaturated fatty‐acid isomerase from Propionibacterium acnes

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Publisher
Wiley
Copyright
Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309106001229
pmid
16511289
Publisher site
See Article on Publisher Site

Abstract

The polyenoic fatty‐acid isomerase from Propionibacterium acnes (PAI) catalyzes the double‐bond isomerization of linoleic acid to conjugated linoleic acid, which is a dairy‐ or meat‐derived fatty acid in the human diet. PAI was overproduced in Escherichia coli and purified to homogeneity as a yellow‐coloured protein. The nature of the bound cofactor was analyzed by absorption and fluorescence spectroscopy. Single crystals of PAI were obtained in two crystal forms. Cubic shaped crystals belong to space group I213, with a unit‐cell parameter of 160.4 Å, and plate‐like crystals belong to the monoclinic space group C2, with unit‐cell parameters a = 133.7, b = 60.8, c = 72.2 Å, β = 115.8°. Both crystal forms contain one molecule per asymmetric unit and diffract to a resolution of better than 2.0 Å. Initial phases were obtained by SIRAS from in‐house data from a cubic crystal that was soaked with an unusually low KI concentration of 0.25 M.

Journal

Acta Crystallographica Section FWiley

Published: Feb 1, 2006

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