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Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm

Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing... An iterative transform method proposed previously for direct phasing of high‐solvent‐content protein crystals is employed for enhancing the molecular‐replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between the template and target structures might be tractable with this modified phasing method. Trial calculations involving three different structures are described to test and illustrate the methodology. The relationship of the approach to PHENIX Phaser‐MR and MR‐Rosetta is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section A: Foundations and Advances Wiley

Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm

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References (69)

Publisher
Wiley
Copyright
Copyright © 2016 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0108-7673
eISSN
1600-5724
DOI
10.1107/S2053273316010731
pmid
27580202
Publisher site
See Article on Publisher Site

Abstract

An iterative transform method proposed previously for direct phasing of high‐solvent‐content protein crystals is employed for enhancing the molecular‐replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between the template and target structures might be tractable with this modified phasing method. Trial calculations involving three different structures are described to test and illustrate the methodology. The relationship of the approach to PHENIX Phaser‐MR and MR‐Rosetta is discussed.

Journal

Acta Crystallographica Section A: Foundations and AdvancesWiley

Published: Sep 1, 2016

Keywords: ; ; ;

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