Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Impaired spinal cord remyelination by long‐term cultured adult porcine olfactory ensheathing cells correlates with altered in vitro phenotypic properties

Impaired spinal cord remyelination by long‐term cultured adult porcine olfactory ensheathing... Radtke C, Lankford KL, Wewetzer K, Imaizumi T, Fodor WL, Kocsis JD. Impaired spinal cord remyelination by long‐term cultured adult porcine olfactory ensheathing cells correlates with altered in vitro phenotypic properties. Xenotransplantation 2010; 17: 71–80. © 2010 John Wiley & Sons A/S. Abstract: Background: Extensive studies in rodents have identified olfactory ensheathing cells (OECs) as promising candidates for cell‐based therapies of spinal cord and peripheral nerve injury. Previously, we demonstrated that short‐term cultured adult porcine OECs can remyelinate the rodent and non‐human primate spinal cord. Here, we studied the impact of the culturing interval on the remyelinating capacity of adult porcine OECs. Methods: Cells were maintained for 1, 2, and 4 to 6 weeks in vitro prior to transplantation into the demyelinated rat spinal cord. Parallel to this, the in vitro phenotypic properties of the OEC preparations used for transplantation were analyzed with regard to morphology, low affinity nerve growth factor receptor (p75NTR) expression and proliferation. Results: We report that prolonged culturing of adult porcine OECs resulted in impaired remyelination of the adult rat spinal cord. Animals receiving transplants of OECs maintained in vitro for 2 weeks displayed significantly less remyelinated axons than those animals that received OEC transplants cultured for 1 week. There was virtually no remyelination after transplantation of OECs cultured for 4 to 6 weeks. The adult porcine OECs displayed a progressive lost of p75NTR‐expression as determined by immunostaining and flow cytometry with time in culture. Conclusions: Taken together, the results indicate that porcine OECs undergo systematic changes with time in culture that result in reduced p75NTR‐expression, decreased proliferation, and reduced remyelinating capability with time in vitro indicating that relatively short term cultures with limited expansion would be required for transplantation studies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Impaired spinal cord remyelination by long‐term cultured adult porcine olfactory ensheathing cells correlates with altered in vitro phenotypic properties

Download PDF
10 pages

Loading next page...
 
/lp/wiley/impaired-spinal-cord-remyelination-by-long-term-cultured-adult-porcine-Y4pfaIMnpe

References (44)

Publisher
Wiley
Copyright
© 2010 John Wiley & Sons A/S
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/j.1399-3089.2009.00562.x
pmid
20149190
Publisher site
See Article on Publisher Site

Abstract

Radtke C, Lankford KL, Wewetzer K, Imaizumi T, Fodor WL, Kocsis JD. Impaired spinal cord remyelination by long‐term cultured adult porcine olfactory ensheathing cells correlates with altered in vitro phenotypic properties. Xenotransplantation 2010; 17: 71–80. © 2010 John Wiley & Sons A/S. Abstract: Background: Extensive studies in rodents have identified olfactory ensheathing cells (OECs) as promising candidates for cell‐based therapies of spinal cord and peripheral nerve injury. Previously, we demonstrated that short‐term cultured adult porcine OECs can remyelinate the rodent and non‐human primate spinal cord. Here, we studied the impact of the culturing interval on the remyelinating capacity of adult porcine OECs. Methods: Cells were maintained for 1, 2, and 4 to 6 weeks in vitro prior to transplantation into the demyelinated rat spinal cord. Parallel to this, the in vitro phenotypic properties of the OEC preparations used for transplantation were analyzed with regard to morphology, low affinity nerve growth factor receptor (p75NTR) expression and proliferation. Results: We report that prolonged culturing of adult porcine OECs resulted in impaired remyelination of the adult rat spinal cord. Animals receiving transplants of OECs maintained in vitro for 2 weeks displayed significantly less remyelinated axons than those animals that received OEC transplants cultured for 1 week. There was virtually no remyelination after transplantation of OECs cultured for 4 to 6 weeks. The adult porcine OECs displayed a progressive lost of p75NTR‐expression as determined by immunostaining and flow cytometry with time in culture. Conclusions: Taken together, the results indicate that porcine OECs undergo systematic changes with time in culture that result in reduced p75NTR‐expression, decreased proliferation, and reduced remyelinating capability with time in vitro indicating that relatively short term cultures with limited expansion would be required for transplantation studies.

Journal

Xenotransplantation – Wiley

Published: Jan 1, 2010

There are no references for this article.