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Identification of HLA‐A2‐restricted immunogenic peptides derived from a xenogenic porcine major histocompatibility complex

Identification of HLA‐A2‐restricted immunogenic peptides derived from a xenogenic porcine major... Background Little information is available regarding the precise swine leukocyte antigen (SLA)‐derived immunogenic peptides that are presented in the context of human HLA molecules. Here, we identified SLA‐derived immunogenic peptides that are presented in association with human HLA‐A2 molecule. Methods The SLA‐derived peptides that bind to HLA‐A*0201, a representative of the A2 supertype, were predicted using a computer‐assisted algorithm. The candidate peptides were synthesized, and the stabilities of complexes formed between peptides and HLA‐A*0201 were compared using major histocompatibility complex (MHC) stabilization assays. The cytotoxic T lymphocyte (CTL)‐inducing activity of the selected peptides was examined in HLA‐A*0201‐transgenic mice. Results Among 15 candidate peptides synthesized, two peptides, peptide‐35 (YLGPDGLLL) and peptide‐43 (TLICHVDSI), were selected to have high affinity and stability with HLA‐A*0201. Examination of the CTL‐inducing activity of the two peptides in HLA‐A*0201‐transgenic mice showed that immunization with peptide‐35, but not peptide‐43, elicited potent CD8‐specific CTL responses. The Peptide‐35 is present in non‐polymorphic α2 domains of 34 SLA‐1 alleles, 18 SLA‐2 alleles, and 1 SLA‐3 allele. Conclusion This study identifies an immunogenic HLA‐A*0201‐restricted epitope derived from the SLA, which may be valuable for the development of epitope‐specific immunoregulation strategies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Identification of HLA‐A2‐restricted immunogenic peptides derived from a xenogenic porcine major histocompatibility complex

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References (40)

Publisher
Wiley
Copyright
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/xen.12119
pmid
25040740
Publisher site
See Article on Publisher Site

Abstract

Background Little information is available regarding the precise swine leukocyte antigen (SLA)‐derived immunogenic peptides that are presented in the context of human HLA molecules. Here, we identified SLA‐derived immunogenic peptides that are presented in association with human HLA‐A2 molecule. Methods The SLA‐derived peptides that bind to HLA‐A*0201, a representative of the A2 supertype, were predicted using a computer‐assisted algorithm. The candidate peptides were synthesized, and the stabilities of complexes formed between peptides and HLA‐A*0201 were compared using major histocompatibility complex (MHC) stabilization assays. The cytotoxic T lymphocyte (CTL)‐inducing activity of the selected peptides was examined in HLA‐A*0201‐transgenic mice. Results Among 15 candidate peptides synthesized, two peptides, peptide‐35 (YLGPDGLLL) and peptide‐43 (TLICHVDSI), were selected to have high affinity and stability with HLA‐A*0201. Examination of the CTL‐inducing activity of the two peptides in HLA‐A*0201‐transgenic mice showed that immunization with peptide‐35, but not peptide‐43, elicited potent CD8‐specific CTL responses. The Peptide‐35 is present in non‐polymorphic α2 domains of 34 SLA‐1 alleles, 18 SLA‐2 alleles, and 1 SLA‐3 allele. Conclusion This study identifies an immunogenic HLA‐A*0201‐restricted epitope derived from the SLA, which may be valuable for the development of epitope‐specific immunoregulation strategies.

Journal

Xenotransplantation – Wiley

Published: Jan 1, 2014

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