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R. Tearle, M. Tange, Z. Zannettino, Marina Katerelos, T. Shinkel, B. Denderen, A. Lonie, I. Lyons, M. Nottle, T. Cox, C. Becker, A. Peura, P. Wigley, Robert Crawford, Allan Robins, Martin Pearse, A. D'apice (1996)
The alpha-1,3-galactosyltransferase knockout mouse. Implications for xenotransplantation.Transplantation, 61 1
L. Airas, Marko Salmi, S. Jalkanen (1993)
Lymphocyte-vascular adhesion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium.Journal of immunology, 151 8
P. Wetzsteon, M. Head, L. Fletcher, W. Lye, D. Norman (1992)
Cytotoxic flow-cytometric crossmatches (flow-tox): a comparison with conventional cytotoxicity crossmatch techniques.Human immunology, 35 2
E. Koren, F. Neethling, Y. Ye, M. Niekrasz, J. Baker, M. Martin, N. Zuhdi, D. Cooper (1992)
Heterogeneity of preformed human antipig xenogeneic antibodies.Transplantation proceedings, 24 2
Cairns Cairns, Karlsson Karlsson, Holgersson Holgersson, Welsh Welsh, Samuelsson Samuelsson (1993)
Confirmation of a major epitope of human natural IgG and IgM anti‐pig antibodies: Terminal galactose α 1,3 galactose.Transplant Proc, 26
B. Collins, W. Parker, J.L PIatt (1994)
Characterization of porcine endothelial cell determinants recognized by human natural antibodiesXenotransplantation, 1
C. Alvarado, A. Cotterell, K. McCurry, B. Collins, J. Magee, Jamie Berthold, J. Logan, J. Platt (1995)
VARIATION IN THE LEVEL OF XENOANTIGEN EXPRESSION IN PORCINE ORGANSTransplantation, 59
H. Vaughan, Ian McKenzie, Mauro Sandrin (1995)
Biochemical studies of pig xenoantigens detected by naturally occurring human antibodies and the galactose alpha(1-3)galactose reactive lectin.Transplantation, 59 1
S. Cooke, R. Hederer, J. Pearson, C. Savage (1995)
CHARACTERIZATION OF HUMAN IgG‐BINDING XENOANTIGENS EXPRESSED BY PORCINE AORTIC ENDOTHELIAL CELLSTransplantation, 60
P. Tuso, D. Cramer, C. Cosenza, Y. Middleton, C. Yasunaga, G. Wu, L. Makowka (1993)
Characterization of human antibodies that are cytotoxic to pig aortic endothelial cells.Transplantation proceedings, 25 1 Pt 1
W. Parker, Dieter Bruno, Z. Holzknecht, J. Platt (1994)
Characterization and affinity isolation of xenoreactive human natural antibodies.Journal of immunology, 153 8
T. Cairns, E. Karlsson, J. Holgersson, D. Taube, K. Welsh, G. Samuelsson (1994)
Confirmation of a major target epitope of human natural IgG and IgM anti-pig antibodies: terminal galactose alpha -1,3-galactose.Transplantation proceedings, 26 3
M. Salmi, S. Jalkanen (1992)
A 90-kilodalton endothelial cell molecule mediating lymphocyte binding in humans.Science, 257 5075
P. Tuso, D. Cramer, Y. Middleton, M. Kearns-Jonker, C. Cosenza, W. Davis, Guo-Du Wu, L. Makowka (1993)
PIG AORTIC ENDOTHELIAL CELL ANTIGENS RECOGNIZED BY HUMAN IgM NATURAL ANTIBODIESTransplantation, 56
M. Pescovitz, J. Lunney, D. Sachs (1984)
Preparation and characterization of monoclonal antibodies reactive with porcine PBL.Journal of immunology, 133 1
A. Ratner, H. Canhui, P. Pepino, J. Sánchez, N. Edwards, J. Watkins, H. Xu, K. Reemtsma, E. Rose, C. Berger (1992)
Lymphocyte xenoantigens recognized by preformed antibodies.Transplantation proceedings, 24 2
M. Kujundžić, E. Koren, F. Neethling, Franko Mitotic, M. Koscec, Tatjana Kujundzic, Mic Martin, D. Cooper (1994)
Variability of anti‐αGal antibodies in human serum and their relation to serum cytotoxicity against pig cellsXenotransplantation, 1
J. Platt, G. Vercellotti, Bonnie Lindman, T. Oegema, F. Bach, A. Dalmasso (1990)
Release of heparan sulfate from endothelial cells. Implications for pathogenesis of hyperacute rejectionThe Journal of Experimental Medicine, 171
U. Galili (1993)
Interaction of the natural anti-Gal antibody with alpha-galactosyl epitopes: a major obstacle for xenotransplantation in humans.Immunology today, 14 10
F. Neethling, E. Koren, Y. Ye, Steven Richards, M. Kujundžić, R. Oriol, D. Cooper (1994)
PROTECTION OF PIG KIDNEY (PK15) CELLS FROM THE CYTOTOXIC EFFECT OF ANTI‐PIG ANTIBODIES BY α‐GALACTOSYL OLIGOSACCHARIDES1Transplantation, 57
J. Platt, Bonnie Lindman, R. Geller, H. Noreen, J. Swanson, A. Dalmasso, F. Bach (1991)
THE ROLE OF NATURAL ANTIBODIES IN THE ACTIVATION OF XENOGENIC ENDOTHELIAL CELLSTransplantation, 52
J. Platt, Z. Holzknecht (1994)
Porcine platelet antigens recognized by human xenoreactive natural antibodies.Transplantation, 57 3
Z. Holzknecht, J. Platt (1995)
Identification of porcine endothelial cell membrane antigens recognized by human xenoreactive natural antibodies.Journal of immunology, 154 9
J. Platt, B. Lindman, H. Chen, S. Spitalnik, F. Bach (1990)
Endothelial cell antigens recognized by xenoreactive human natural antibodies.Transplantation, 50 5
H. Vaughan, P. Dabkowski, I. Mckenzie, M. Sandrin (1993)
Biochemical analysis of pig xenoantigens detected by human antibodies.Transplantation proceedings, 25 5
Abstract: The shortage of human donors for clinical transplantation has led to a serious consideration of the use of non‐human species as organ donors. The major barrier to the clinical use of xenografts from species such as the pig in human transplantation has been the aggressive nature of the immune‐mediated rejection of the graft. We have recently identified the molecular weights of several endothelial cell surface proteins that may be targets of human antibody‐mediated responses to pig aortic endothelial cells (PAEC). In this series of experiments, we produced a panel of rat monoclonal antibodies (Mabs) to PAEC in an effort to identify Mabs that detect pig xenoantigens. Mabs were selected based on flow cytometric binding to PAEC, pig platelets, and various pig cell lines, including a pig kidney cell line (LLC‐PK1) reported to react with human natural antibodies (HNA). Eleven of the eighty‐three antibodies produced were cytotoxic for PAEC. Six of the cytotoxic clones recognized a 44 kDa protein and two of the clones recognized a 115 kDa protein expressed on the surface of PAEC. Since PAEC target antigens recognized by human natural antibodies include both 115 and 44 kDa antigens, these Mab clones were selected for further study. Several distinct patterns of tissue reactivity were demonstrated within this group of antibodies by immunohistochemical analysis; however all monoclonal antibodies were highly reactive with endothelial cells in all tissues examined. Two monoclonal antibodies recognizing antigens that are highly expressed on pig endothelial cells (92–98%) and pig platelets (74–92%), but moderately expressed on pig splenocytes (33–38%), were capable of reproducibly blocking 48–53% of human IgM binding to pig endothelial cells when analyzed with flow cytometry. This data suggests that these Mabs may recognize epitopes of potential significance in the human‐to‐pig xenograft reaction.
Xenotransplantation – Wiley
Published: Nov 1, 1996
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