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Humidity control as a strategy for lattice optimization applied to crystals of HLA‐A*1101 complexed with variant peptides from dengue virus

Humidity control as a strategy for lattice optimization applied to crystals of HLA‐A*1101... T‐cell recognition of the antigenic peptides presented by MHC class I molecules normally triggers protective immune responses, but can result in immune enhancement of disease. Cross‐reactive T‐cell responses may underlie immunopathology in dengue haemorrhagic fever. To analyze these effects at the molecular level, the functional MHC class I molecule HLA‐A*1101 was crystallized bound to six naturally occurring peptide variants from the dengue virus NS3 protein. The crystals contained high levels of solvent and required optimization of the cryoprotectant and dehydration protocols for each complex to yield well ordered diffraction, a process that was facilitated by the use of a free‐mounting system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Humidity control as a strategy for lattice optimization applied to crystals of HLA‐A*1101 complexed with variant peptides from dengue virus

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References (15)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309107013693
pmid
17565177
Publisher site
See Article on Publisher Site

Abstract

T‐cell recognition of the antigenic peptides presented by MHC class I molecules normally triggers protective immune responses, but can result in immune enhancement of disease. Cross‐reactive T‐cell responses may underlie immunopathology in dengue haemorrhagic fever. To analyze these effects at the molecular level, the functional MHC class I molecule HLA‐A*1101 was crystallized bound to six naturally occurring peptide variants from the dengue virus NS3 protein. The crystals contained high levels of solvent and required optimization of the cryoprotectant and dehydration protocols for each complex to yield well ordered diffraction, a process that was facilitated by the use of a free‐mounting system.

Journal

Acta Crystallographica Section FWiley

Published: May 1, 2007

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