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Human complement‐activating immunoglobulin (Ig)G3 antibodies are essential for porcine endothelial cell activation

Human complement‐activating immunoglobulin (Ig)G3 antibodies are essential for porcine... Sæthre M, Lea T, Borgen MS, Fiane AE, Michaelsen TE, Thorsby E, Haraldsen G, Mollnes TE. Human complement‐activating immunoglobulin (Ig)G3 antibodies are essential for porcine endothelial cell activation. Xenotransplantation 2006; 13: 215–223. © Blackwell Munksgaard, 2006 Abstract: Background: Complement‐activating naturally occurring anti‐porcine endothelial cell antibodies (Abs) are responsible for hyperacute rejection in porcine‐to‐primate transplantation, whereas the role of complement in acute vascular rejection, characterized by type II endothelial cell activation, is less well understood. We previously demonstrated a correlation between porcine type II endothelial cell activation, as detected by E‐selectin expression, and human immunoglobulin (Ig)G3 anti‐Galα1‐3Gal (Gal) Abs, which was not seen for IgG1, IgG2 or IgG4. The present study was undertaken to investigate whether there is a causal relationship between human anti‐porcine IgG3 Abs and porcine endothelial cell activation. Methods: IgG3 was isolated employing a Protein A column to 98.3% purity. Porcine endothelial cells were incubated with isolated human IgG3 or the combination of IgG1, IgG2 and IgG4. E‐selectin expression and complement activation were investigated by flow cytometry and Western blotting, respectively. Results: Purified IgG3, in contrast to the other IgG subclasses, induced a substantial increase in E‐selectin expression. This activation was accompanied by complement activation as detected by C3 cleavage, and was abolished by heat inactivation or by adding the complement inhibitor FUT‐175. Depletion of anti‐Gal Abs reduced E‐selectin expression by 60%, consistent with the presence of complement‐activating anti‐porcine non‐Gal Abs of the IgG3 subclass. Conclusions: Collectively, these data strengthen the hypothesis that human anti‐porcine endothelial cell Abs of the IgG3 subclass are essential for endothelial cell activation in porcine‐to‐human species grafts and demonstrate such activation to be partly independent of Gal epitopes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Human complement‐activating immunoglobulin (Ig)G3 antibodies are essential for porcine endothelial cell activation

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References (62)

Publisher
Wiley
Copyright
Copyright © 2006
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/j.1399-3089.2006.00289.x
pmid
16756564
Publisher site
See Article on Publisher Site

Abstract

Sæthre M, Lea T, Borgen MS, Fiane AE, Michaelsen TE, Thorsby E, Haraldsen G, Mollnes TE. Human complement‐activating immunoglobulin (Ig)G3 antibodies are essential for porcine endothelial cell activation. Xenotransplantation 2006; 13: 215–223. © Blackwell Munksgaard, 2006 Abstract: Background: Complement‐activating naturally occurring anti‐porcine endothelial cell antibodies (Abs) are responsible for hyperacute rejection in porcine‐to‐primate transplantation, whereas the role of complement in acute vascular rejection, characterized by type II endothelial cell activation, is less well understood. We previously demonstrated a correlation between porcine type II endothelial cell activation, as detected by E‐selectin expression, and human immunoglobulin (Ig)G3 anti‐Galα1‐3Gal (Gal) Abs, which was not seen for IgG1, IgG2 or IgG4. The present study was undertaken to investigate whether there is a causal relationship between human anti‐porcine IgG3 Abs and porcine endothelial cell activation. Methods: IgG3 was isolated employing a Protein A column to 98.3% purity. Porcine endothelial cells were incubated with isolated human IgG3 or the combination of IgG1, IgG2 and IgG4. E‐selectin expression and complement activation were investigated by flow cytometry and Western blotting, respectively. Results: Purified IgG3, in contrast to the other IgG subclasses, induced a substantial increase in E‐selectin expression. This activation was accompanied by complement activation as detected by C3 cleavage, and was abolished by heat inactivation or by adding the complement inhibitor FUT‐175. Depletion of anti‐Gal Abs reduced E‐selectin expression by 60%, consistent with the presence of complement‐activating anti‐porcine non‐Gal Abs of the IgG3 subclass. Conclusions: Collectively, these data strengthen the hypothesis that human anti‐porcine endothelial cell Abs of the IgG3 subclass are essential for endothelial cell activation in porcine‐to‐human species grafts and demonstrate such activation to be partly independent of Gal epitopes.

Journal

Xenotransplantation – Wiley

Published: May 1, 2006

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