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Human CD8 responder cells can be directly activated to proliferate and to produce IL‐2 following stimulation by allogeneic, but not by xenogeneic, porcine blood mononuclear cells

Human CD8 responder cells can be directly activated to proliferate and to produce IL‐2 following... Abstract: In order to determine the precise nature of human T lymphocytes reactivity against porcine stimulator cells, purified CD4+ and CD8+ human peripheral T lymphocytes have been tested for their responsiveness against porcine stimulator cells. In a xenogeneic mixed lymphocyte reaction (MLR), CD4+ T cells were capable of proliferating as a result of the recognition of porcine peripheral blood lymphocytes (PBL), whereas CD8+ T cells were unresponsive. A proliferative response of CD8+ T cells could be restored by treatment with human IL‐2, but not by IL‐lα, IL‐lβ, or IL‐6. Production of IL‐2 was not detected in the xenostimulated CD8+ responder cells, nor could IL‐2 production be restored by the addition of IL‐lα, IL‐1β, or IL‐6. The presence of human CD4+ responder cells was crucial both for a xenoproliferative response and for IL‐2 synthesis. However, when the expression of the IL‐2 receptor (CD25) on the CD8+ T cells was analyzed, no difference was detected between xenostimulated and allostimulated CD8+ T cells. When the development of cytotoxic T cells in xenogeneic and allogeneic MLRs was compared, the cytotoxic activity exhibited by purified CD8+ T cells in xenogeneic MLR was significantly lower than that in the allogeneic combination. In the xenogeneic combination, exogenous IL‐2 reconstituted the cytotoxicity by purified CD8+ T cells; however, IL‐lα, IL‐lβ, or IL‐6 did not. Our results show that purified human CD4+ T cells respond directly against pig PBMCs, whereas purified CD8+ T cells do not. Furthermore, responsiveness in CD8+ T cells is completely restored by the addition of human IL‐2. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Human CD8 responder cells can be directly activated to proliferate and to produce IL‐2 following stimulation by allogeneic, but not by xenogeneic, porcine blood mononuclear cells

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References (26)

Publisher
Wiley
Copyright
© 1997 Munksgaard
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/j.1399-3089.1997.tb00171.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: In order to determine the precise nature of human T lymphocytes reactivity against porcine stimulator cells, purified CD4+ and CD8+ human peripheral T lymphocytes have been tested for their responsiveness against porcine stimulator cells. In a xenogeneic mixed lymphocyte reaction (MLR), CD4+ T cells were capable of proliferating as a result of the recognition of porcine peripheral blood lymphocytes (PBL), whereas CD8+ T cells were unresponsive. A proliferative response of CD8+ T cells could be restored by treatment with human IL‐2, but not by IL‐lα, IL‐lβ, or IL‐6. Production of IL‐2 was not detected in the xenostimulated CD8+ responder cells, nor could IL‐2 production be restored by the addition of IL‐lα, IL‐1β, or IL‐6. The presence of human CD4+ responder cells was crucial both for a xenoproliferative response and for IL‐2 synthesis. However, when the expression of the IL‐2 receptor (CD25) on the CD8+ T cells was analyzed, no difference was detected between xenostimulated and allostimulated CD8+ T cells. When the development of cytotoxic T cells in xenogeneic and allogeneic MLRs was compared, the cytotoxic activity exhibited by purified CD8+ T cells in xenogeneic MLR was significantly lower than that in the allogeneic combination. In the xenogeneic combination, exogenous IL‐2 reconstituted the cytotoxicity by purified CD8+ T cells; however, IL‐lα, IL‐lβ, or IL‐6 did not. Our results show that purified human CD4+ T cells respond directly against pig PBMCs, whereas purified CD8+ T cells do not. Furthermore, responsiveness in CD8+ T cells is completely restored by the addition of human IL‐2.

Journal

Xenotransplantation – Wiley

Published: May 1, 1997

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