Access the full text.
Sign up today, get DeepDyve free for 14 days.
Allen Murray, Mehran Khodedoust, Jordan Pober, Alfred Bothwell (1994)
Porcine aortic endothelial cells activate human T cells: direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28.Immunity, 1 1
Kenneth Yamada, D. Sachs, H. Dersimonian (1995)
Human anti-porcine xenogeneic T cell response. Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition.Journal of immunology, 155 11
Seebach Seebach, Yamada Yamada, McMorrow McMorrow, Sachs Sachs, DerSimonian DerSimonian (1996)
Xenogeneic human anti‐pig cytotoxicity mediated by natural killer (NK) cellsXenotransplant
Yamada Yamada, Sachs Sachs, DerSimonian DerSimonian (1995)
The human anti‐porcine xenogeneic T‐cell response: Evidence for allelic specificity of MLR and for both direct and indirect path ways of recognitionJ Immunol, 155
M. Sandrin, I. Mckenzie (1994)
Galα(1,3)Gal, the Major Xenoantigen(s) Recognised in Pigs by Human Natural AntibodiesImmunological Reviews, 141
M. Elsen, M. Soares, D. Latinne, A. Cornet, H. Bazin (1993)
Role of activated natural killer and CD4+, CD8+ cells in the cellular rejection of a discordant xenograft.Transplantation proceedings, 25 1 Pt 1
P. Martin, G. Longton, J. Ledbetter, W. Newman, MP Braun, P. Beatty, J. Hansen (1983)
Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50.Journal of immunology, 131 1
J. Platt, G. Vercellotti, A. Dalmasso, A. Dalmasso, A. Matas, R. Bolman, J. Najarian, F. Bach, F. Bach (1990)
Transplantation of discordant xenografts: a review of progress.Immunology today, 11 12
A. Cunningham (1994)
Future developments in transplantation: Cellular aspects of xenotransplantationTransplant Immunology, 2
R. Moses, R. Pierson, H. Winn, H. Auchincloss (1990)
Xenogeneic proliferation and lymphokine production are dependent on CD4+ helper T cells and self antigen-presenting cells in the mouseThe Journal of Experimental Medicine, 172
J. Fryer, J. Leventhal, A. Dalmasso, Sally Chen, Pamela Simone, J. Goswitz, N. Reinsmoen, A. Matas (1995)
Beyond Hyperacute Rejection: Accelerated Rejection In A Discordant Xenograft Model By Adoptive Transfer Of Specific Cell SubsetsTransplantation, 59
Ozato Ozato, Mayer Mayer, Sachs Sachs (1982)
Monoclonal antibodies to mouse major histocompatibility complex antigensTransplantation, 34
B. Alter, Fritz Bach (1990)
Cellular basis of the proliferative response of human T cells to mouse xenoantigensThe Journal of Experimental Medicine, 171
Sachs (1994)
The pig as a potential xenograft donorPath Biol, 42
D. Sachs, T. Sablinski (1995)
Tolerance across discordant xenogeneic barriersXenotransplantation, 2
David Sachs (1994)
The pig as a potential xenograft donor.Veterinary immunology and immunopathology, 43 1-3
A. Rosengard, N. Cary, J. Horsley, C. Belcher, G. Langford, E. Cozzi, J. Wallwork, D. White (1995)
Endothelial expression of human decay accelerating factor in transgenic pig tissue: a potential approach for human complement inactivation in discordant xenografts.Transplantation proceedings, 27 1
D. Sachs (1994)
The pig as a xenograft donor.Pathologie-biologie, 42 3
J. Seebach, Kazuhiko Yamada, I. McMorrow, D. Sachs, H. Dersimonian (1996)
Xenogeneic human anti‐pig cytotoxicity mediated by activated natural killer cellsXenotransplantation, 3
P. Lucas, G. Shearer, S. Neudorf, R. Gress (1990)
The human antimurine xenogeneic cytotoxic response. I. Dependence on responder antigen-presenting cells.Journal of immunology, 144 12
Cunningham Cunningham (1994)
Cellular aspects of xenotransplantationTranspl Immunol, 2
K. McCurry, D. Kooyman, C. Alvarado, A. Cotterell, Michael Martin, J. Logan, J. Platt (1995)
Human complement regulatory proteins protect swine-to-primate cardiac xenografts from humoral injuryNature Medicine, 1
M. Blakely, W. Werf, M. Berndt, A. Dalmasso, F. Bach, W. Hancock (1994)
Activation of intragraft endothelial and mononuclear cells during discordant xenograft rejection.Transplantation, 58 10
A. Kirk, R. Li, M. Kinch, K. Abernethy, C. Doyle, R. Bollinger (1993)
The human antiporcine cellular repertoire. In vitro studies of acquired and innate cellular responsiveness.Transplantation, 55 4
K. Ozato, N. Mayer, D. Sachs (1982)
MONOCLONAL ANTIBODIES TO MOUSE MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS: IV. A SERIES OF HYBRIDOMA CLONES PRODUCING ANTI‐H-2d ANTIBODIES AND AN EXAMINATION OF EXPRESSION OF H‐2d ANTIGENS ON THE SURFACE OF THESE CELLSTransplantation, 34
Abstract: Miniature swine have a variety of advantages as potential donors for human xenotransplantation, including size, physiological similarities, and breeding characteristics. To investigate the nature of the human anti‐pig xenogeneic cellular response, we performed standard 51Cr‐release cell‐mediated lympholysis (CML) experiments. The major histocompatibility complex (MHC) allele specificity of the xenogeneic cellular response was tested on porcine target cells of three distinct homozygous MHC haplotypes (SLAaa, SLACC, SLAdd) and three intra‐MHC recombinant haplotypes (SLAjj, SLAgg, SLAkk), obtained from our herd of partially inbred miniature swine. After stimulation with irradiated porcine peripheral blood mononuclear cells (PBMC) of SLAaa haplotype, a strong nonspecific cytotoxic response of the bulk culture against xenogeneic targets of all three haplotypes was observed. However, SLA allele specificity could be demonstrated after T cell enrichment, and mapping experiments revealed predominantly SLA class I restriction of xenoreactive cytotoxic T lymphocytes (CTLs), although some class II restriction was also observed. The experiments were repeated in the presence of anti‐T cell monoclonal antibodies, anti‐CD3 (OKT3), anti‐CD2 (35.1), anti‐CD4 (OKT4), or anti‐CD 8 (OKT8). The bulk xenogeneic CML was not inhibited by any of the anti‐T cell antibodies tested. However, after T cell‐enrichment, lysis of porcine targets was significantly inhibited by anti‐CD3 or anti‐CD8 antibody and partially inhibited by anti‐CD2 antibody. In comparable assays, the human allogeneic CML was blocked by anti‐CD3 and anti‐CD8, but not by anti‐CD2 or anti‐CD4 antibodies. Finally, the cytotoxic activity of A3b3, a human CD4+ T‐cell clone, was tested. A3b3 lysed xenogeneic targets of SLAaa haplotype, but not SLACC or allogeneic targets, and was inhibited by anti‐CD4, anti‐CD2, and anti‐CD3 antibodies, but not by anti‐CD8. With the aid of intra‐MHC recombinant haplotypes, the xenogeneic CML reactivity of A3b3 was mapped to SLA class II, suggesting direct xenogeneic recognition of porcine MHC class II antigens by human T cells. Thus, the human anti‐pig cell‐mediated cytotoxic response is similar in magnitude to comparable allogeneic responses, and involves both SLA class I and class II restricted T‐cell mediated cytotoxicity, as well as additional nonspecific killing, possibly by NK cells.
Xenotransplantation – Wiley
Published: May 1, 1996
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.