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Highly Efficient Agrobacterium‐Mediated Transformation of Potato (Solanum tuberosum) and Production of Transgenic Microtubers

Highly Efficient Agrobacterium‐Mediated Transformation of Potato (Solanum tuberosum) and... The following method enables the rapid production of transgenic potato plants and microtubers for gene validation and expression, or promoter studies. The method is highly efficient, with reproducible transformation efficiencies of at least 50% to 60% with potato cv. Desiree, and can produce transgenic microtubers within 6 months of initiation of the experiment. Microtubers are produced in the absence of hormones, giving an in vitro gene testing system broadly analogous to the natural state. © 2018 by John Wiley & Sons, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Protocols in Plant Biology Wiley

Highly Efficient Agrobacterium‐Mediated Transformation of Potato (Solanum tuberosum) and Production of Transgenic Microtubers

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References (14)

Publisher
Wiley
Copyright
© 2018 John Wiley & Sons, Inc.
ISSN
2379-8068
eISSN
2379-8068
DOI
10.1002/cppb.20065
pmid
30040252
Publisher site
See Article on Publisher Site

Abstract

The following method enables the rapid production of transgenic potato plants and microtubers for gene validation and expression, or promoter studies. The method is highly efficient, with reproducible transformation efficiencies of at least 50% to 60% with potato cv. Desiree, and can produce transgenic microtubers within 6 months of initiation of the experiment. Microtubers are produced in the absence of hormones, giving an in vitro gene testing system broadly analogous to the natural state. © 2018 by John Wiley & Sons, Inc.

Journal

Current Protocols in Plant BiologyWiley

Published: Mar 1, 2018

Keywords: ; ; ;

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