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High‐resolution structure of a Kazal‐type serine protease inhibitor from the dengue vector Aedes aegypti

High‐resolution structure of a Kazal‐type serine protease inhibitor from the dengue vector Aedes... Blood‐feeding exoparasites are rich sources of protease inhibitors, and the mosquito Aedes aegypti, which is a vector of Dengue virus, Yellow fever virus, Chikungunya virus and Zika virus, is no exception. AaTI is a single‐domain, noncanonical Kazal‐type serine proteinase inhibitor from A. aegypti that recognizes both digestive trypsin‐like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. Here, the 1.4 Å resolution crystal structure of AaTI is reported from extremely tightly packed crystals (∼22% solvent content), revealing the structural determinants for the observed inhibitory profile of this molecule. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

High‐resolution structure of a Kazal‐type serine protease inhibitor from the dengue vector Aedes aegypti

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Publisher
Wiley
Copyright
Copyright © 2017 Wiley Subscription Services
ISSN
2053-230X
eISSN
2053-230X
DOI
10.1107/S2053230X17010007
pmid
28777090
Publisher site
See Article on Publisher Site

Abstract

Blood‐feeding exoparasites are rich sources of protease inhibitors, and the mosquito Aedes aegypti, which is a vector of Dengue virus, Yellow fever virus, Chikungunya virus and Zika virus, is no exception. AaTI is a single‐domain, noncanonical Kazal‐type serine proteinase inhibitor from A. aegypti that recognizes both digestive trypsin‐like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. Here, the 1.4 Å resolution crystal structure of AaTI is reported from extremely tightly packed crystals (∼22% solvent content), revealing the structural determinants for the observed inhibitory profile of this molecule.

Journal

Acta Crystallographica Section FWiley

Published: Jan 1, 2017

Keywords: ; ; ; ; ; ; ; ; ;

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