Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Genetic studies on human lymphoblastoid cell lines: isozyme and cytogenetic heterogeneity in a cell line, with evidence for localization of the Pep A locus in man

Genetic studies on human lymphoblastoid cell lines: isozyme and cytogenetic heterogeneity in a... Genetic studies on human lymphoblastoid cell lines : isozyme and cytogenetic heterogeneity in a cell line, with evidence for localization of the Pep A locus in man BY ELIZABETH ARTHUR, C. M. STEEL AND H. J. EVANS Medical Research Council, Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh SUSAN POVEY, B. WATSON AND HARRY HARRIS Medical Research Council, Human Biochemical Genetics Unit, Galton Laboratory, University College London I n a series of experiments previously described (Povey et al. 1973) four possible mutations were found among the cloned progeny of a human male lymphoblastoid cell line (F137) after exposure to the chemical mutagen MNNG. We now present data from a further 133 clones (60 mutagen-treated, 73 controls) from the same line. On both cytogenetic and isosyme analysis there is evidence for genetic heterogeneity in the parent line due to the spontaneous emergence of mutant clones under normal culture conditions. This has important implications for the use of such lines in genetic experiments. Examination of the data with particular reference to the enzyme Peptidase A provides strong evidence for localization of the human Pep A structural locus on the qter region of chromosome 18 and gives the first indication http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Human Genetics Wiley

Genetic studies on human lymphoblastoid cell lines: isozyme and cytogenetic heterogeneity in a cell line, with evidence for localization of the Pep A locus in man

Loading next page...
 
/lp/wiley/genetic-studies-on-human-lymphoblastoid-cell-lines-isozyme-and-YhQ0TogdiY

References (22)

Publisher
Wiley
Copyright
Copyright © 1975 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0003-4800
eISSN
1469-1809
DOI
10.1111/j.1469-1809.1975.tb00105.x
Publisher site
See Article on Publisher Site

Abstract

Genetic studies on human lymphoblastoid cell lines : isozyme and cytogenetic heterogeneity in a cell line, with evidence for localization of the Pep A locus in man BY ELIZABETH ARTHUR, C. M. STEEL AND H. J. EVANS Medical Research Council, Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh SUSAN POVEY, B. WATSON AND HARRY HARRIS Medical Research Council, Human Biochemical Genetics Unit, Galton Laboratory, University College London I n a series of experiments previously described (Povey et al. 1973) four possible mutations were found among the cloned progeny of a human male lymphoblastoid cell line (F137) after exposure to the chemical mutagen MNNG. We now present data from a further 133 clones (60 mutagen-treated, 73 controls) from the same line. On both cytogenetic and isosyme analysis there is evidence for genetic heterogeneity in the parent line due to the spontaneous emergence of mutant clones under normal culture conditions. This has important implications for the use of such lines in genetic experiments. Examination of the data with particular reference to the enzyme Peptidase A provides strong evidence for localization of the human Pep A structural locus on the qter region of chromosome 18 and gives the first indication

Journal

Annals of Human GeneticsWiley

Published: Jul 1, 1975

There are no references for this article.