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- Publisher
- Wiley
- Copyright
- Copyright © 2017 Wiley Subscription Services
- ISSN
- 2053-230X
- eISSN
- 2053-230X
- DOI
- 10.1107/S2053230X17008214
- pmid
- 28695849
- Publisher site
- See Article on Publisher Site
Abstract
NisI is a lantibiotic‐binding lipoprotein that is specific for nisin. Nisin‐producing microorganisms use NisI as an immunity protein for self‐protection against nisin. Here, the purification, crystallization and preliminary X‐ray diffraction of full‐length NisI from Lactobacillus lactis in a lipid‐free form (NisI22‐C) are reported. Importantly, reductive methylation of the lysine residues in NisI22‐C was essential for initial crystallization. Only methylated NisI22‐C crystallized. The optimized crystals of methylated NisI22‐C were grown in 30–40 mM ammonium sulfate, 0.1 M sodium acetate pH 4.6, 16–18% PEG 4000 at 295 K and diffracted to 1.9 Å resolution. The crystal belonged to space group P212121, with unit‐cell parameters a = 45.99, b = 76.67, c = 76.39 Å, α = β = γ = 90.0°. Assuming the presence of one molecule in the asymmetric unit, the estimated Matthews coefficient (VM) is 2.58 Å3 Da−1 and the estimated solvent content is 52.3%.
Journal
Acta Crystallographica Section F – Wiley
Published: Jan 1, 2017
Keywords: ; ; ; ;