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Filament‐like DREP4 CIDE domain: characterization and preliminary X‐ray crystallographic studies

Filament‐like DREP4 CIDE domain: characterization and preliminary X‐ray crystallographic studies DREP4 is a nuclease from fruit fly that is involved in apoptotic DNA fragmentation. DREP4 contains a conserved CIDE domain that acts as a protein‐interaction module and is critical for its function. In this study, it was found that DREP4 CIDE domains form filament‐like structures in solution. The length of the highly ordered filament‐like structure is dependent on the salt concentration. By adjusting the salt concentration the DREP4 CIDE domain could be crystallized, and X‐ray diffraction data were collected to a resolution of 1.9 Å. The crystals were found to belong to the orthorhombic space group P212121, with unit‐cell parameters a = 53.08, b = 76.58, c = 174.59 Å. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Filament‐like DREP4 CIDE domain: characterization and preliminary X‐ray crystallographic studies

Acta Crystallographica Section F , Volume 73 (8) – Jan 1, 2017

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Publisher
Wiley
Copyright
Copyright © 2017 Wiley Subscription Services
ISSN
2053-230X
eISSN
2053-230X
DOI
10.1107/S2053230X1701069X
pmid
28777092
Publisher site
See Article on Publisher Site

Abstract

DREP4 is a nuclease from fruit fly that is involved in apoptotic DNA fragmentation. DREP4 contains a conserved CIDE domain that acts as a protein‐interaction module and is critical for its function. In this study, it was found that DREP4 CIDE domains form filament‐like structures in solution. The length of the highly ordered filament‐like structure is dependent on the salt concentration. By adjusting the salt concentration the DREP4 CIDE domain could be crystallized, and X‐ray diffraction data were collected to a resolution of 1.9 Å. The crystals were found to belong to the orthorhombic space group P212121, with unit‐cell parameters a = 53.08, b = 76.58, c = 174.59 Å.

Journal

Acta Crystallographica Section FWiley

Published: Jan 1, 2017

Keywords: ; ; ;

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