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Expression, purification, crystallization and preliminary X‐ray analysis of NAD(P)H‐dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver

Expression, purification, crystallization and preliminary X‐ray analysis of NAD(P)H‐dependent... An NAD(P)H‐dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting‐drop vapour‐diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit‐cell parameters a = 104.26, b = 81.32, c = 77.27 Å, β = 119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Expression, purification, crystallization and preliminary X‐ray analysis of NAD(P)H‐dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver

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References (16)

Publisher
Wiley
Copyright
International Union of Crystallography, 2012
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309112046453
pmid
23192050
Publisher site
See Article on Publisher Site

Abstract

An NAD(P)H‐dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting‐drop vapour‐diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit‐cell parameters a = 104.26, b = 81.32, c = 77.27 Å, β = 119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%.

Journal

Acta Crystallographica Section FWiley

Published: Dec 1, 2012

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