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References (16)
-
Xueyan Ma, S. Panjikar, J. Koepke, Elke Loris, J. Stöckigt (2006)
The Structure of Rauvolfia serpentina Strictosidine Synthase Is a Novel Six-Bladed β-Propeller Fold in Plant Proteins[W]The Plant Cell Online, 18
-
T. Grothe, R. Lenz, T. Kutchan (2001)
Molecular Characterization of the Salutaridinol 7-O-Acetyltransferase Involved in Morphine Biosynthesis in Opium Poppy Papaver somniferum *The Journal of Biological Chemistry, 276
-
B. Matthews (1968)
Solvent content of protein crystals.Journal of molecular biology, 33 2
-
S. Marillonnet, C. Thoeringer, R. Kandzia, V. Klimyuk, Y. Gleba (2005)
Systemic Agrobacterium tumefaciens–mediated transfection of viral replicons for efficient transient expression in plantsNature Biotechnology, 23
-
Xueyan Ma, J. Koepke, S. Panjikar, G. Fritzsch, J. Stöckigt (2005)
Crystal Structure of Vinorine Synthase, the First Representative of the BAHD Superfamily*Journal of Biological Chemistry, 280
-
Xueyan Ma, J. Koepke, G. Fritzsch, Ralf Diem, T. Kutchan, H. Michel, J. Stöckigt (2004)
Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family.Biochimica et biophysica acta, 1702 1
-
Wojciech Rypniewski, Hazel Holden, I. Rayment (1993)
Structural consequences of reductive methylation of lysine residues in hen egg white lysozyme: an X-ray analysis at 1.8-A resolution.Biochemistry, 32 37
-
I. Gavidia, P. Pérez-Bermúdez, H. Seitz (2002)
Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves.European journal of biochemistry, 269 12
-
A. Vagin, A. Teplyakov (1997)
MOLREP: an Automated Program for Molecular ReplacementJournal of Applied Crystallography, 30
-
K. Kavanagh, M. Klimacek, B. Nidetzky, David Wilson (2002)
The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis.Biochemistry, 41 28
-
(2004)
Biochim -
Benoit St-Pierre, Pierre Laflamme, A. Alarco, Vincenzo D, E. Luca (1998)
The terminal O-acetyltransferase involved in vindoline biosynthesis defines a new class of proteins responsible for coenzyme A-dependent acyl transfer.The Plant journal : for cell and molecular biology, 14 6
-
M. Ruppert, J. Woll, A. Giritch, Ezzat Genady, Xueyan Ma, J. Stöckigt (2005)
Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression systemPlanta, 222
-
W. Kabsch (1993)
Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constantsJournal of Applied Crystallography, 26
-
Leif Barleben, Xueyan Ma, J. Koepke, G. Peng, H. Michel, J. Stöckigt (2005)
Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons.Biochimica et biophysica acta, 1747 1
-
A. Ehrensberger, David Wilson (2004)
Structural and catalytic diversity in the two family 11 aldo-keto reductases.Journal of molecular biology, 337 3
- Publisher
- Wiley
- Copyright
- Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S174430910605041X
- pmid
- 17142919
- Publisher site
- See Article on Publisher Site
Abstract
Perakine reductase (PR) is a novel member of the aldo‐keto reductase enzyme superfamily from higher plants. PR from the plant Rauvolfia serpentina is involved in the biosynthesis of monoterpenoid indole alkaloids by performing NADPH‐dependent reduction of perakine, yielding raucaffrinoline. However, PR can also reduce cinnamic aldehyde and some of its derivatives. After heterologous expression of a triple mutant of PR in Escherichia coli, crystals of the purified and methylated enzyme were obtained by the hanging‐drop vapour‐diffusion technique at 293 K with 100 mM sodium citrate pH 5.6 and 27% PEG 4000 as precipitant. Crystals belong to space group C2221 and diffract to 2.0 Å, with unit‐cell parameters a = 58.9, b = 93.0, c = 143.4 Å.
Journal
Acta Crystallographica Section F – Wiley
Published: Dec 1, 2006