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Expression, purification, crystallization and preliminary X‐ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae

Expression, purification, crystallization and preliminary X‐ray diffraction analysis of... There are three thioredoxin isoforms in the yeast Saccharomyces cerevisiae: two cytosolic/nuclear thioredoxins, Trx1 and Trx2, and one mitochondrial thioredoxin, Trx3. In the present work, S. cerevisiae Trx3 overexpressed in Escherichia coli was purified and crystallized. The Trx3 crystals were obtained by the hanging‐drop vapour‐diffusion method. A data set diffracting to 2.0 Å resolution was collected from a single crystal. The crystal belongs to space group P31, with unit‐cell parameters a = b = 49.57, c = 94.55 Å, α = β = 90, γ = 120°. The asymmetric unit is assumed to contain two subunits of Trx3, with a VM value of 2.62 Å3 Da−1 and a solvent content of 53%. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Expression, purification, crystallization and preliminary X‐ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae

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References (5)

Publisher
Wiley
Copyright
Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309106041467
pmid
17077505
Publisher site
See Article on Publisher Site

Abstract

There are three thioredoxin isoforms in the yeast Saccharomyces cerevisiae: two cytosolic/nuclear thioredoxins, Trx1 and Trx2, and one mitochondrial thioredoxin, Trx3. In the present work, S. cerevisiae Trx3 overexpressed in Escherichia coli was purified and crystallized. The Trx3 crystals were obtained by the hanging‐drop vapour‐diffusion method. A data set diffracting to 2.0 Å resolution was collected from a single crystal. The crystal belongs to space group P31, with unit‐cell parameters a = b = 49.57, c = 94.55 Å, α = β = 90, γ = 120°. The asymmetric unit is assumed to contain two subunits of Trx3, with a VM value of 2.62 Å3 Da−1 and a solvent content of 53%.

Journal

Acta Crystallographica Section F – Wiley

Published: Nov 1, 2006

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