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Expression, purification, crystallization and preliminary X‐ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles

Expression, purification, crystallization and preliminary X‐ray crystallographic studies of... Recombinant hepatitis B virus core proteins dimerize to form building blocks that are capable of self‐assembly into a capsid. A core capsid protein dimer (CPD) linked to a green fluorescent protein variant, EGFP, at the C‐terminus has been designed. The recombinant fusion CPD was expressed in Escherichia coli, assembled into virus‐like particles (VLPs), purified and crystallized. The single crystal diffracted to 2.15 Å resolution and belonged to the cubic space group F432, with unit‐cell parameters a = b = c = 219.7 Å. The fusion proteins assembled into icosahedral VLPs in aqueous solution, but were rearranged into octahedral symmetry through the crystal‐packing process under the crystallization conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Expression, purification, crystallization and preliminary X‐ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles

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References (27)

Publisher
Wiley
Copyright
International Union of Crystallography, 2013
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309112052074
pmid
23385760
Publisher site
See Article on Publisher Site

Abstract

Recombinant hepatitis B virus core proteins dimerize to form building blocks that are capable of self‐assembly into a capsid. A core capsid protein dimer (CPD) linked to a green fluorescent protein variant, EGFP, at the C‐terminus has been designed. The recombinant fusion CPD was expressed in Escherichia coli, assembled into virus‐like particles (VLPs), purified and crystallized. The single crystal diffracted to 2.15 Å resolution and belonged to the cubic space group F432, with unit‐cell parameters a = b = c = 219.7 Å. The fusion proteins assembled into icosahedral VLPs in aqueous solution, but were rearranged into octahedral symmetry through the crystal‐packing process under the crystallization conditions.

Journal

Acta Crystallographica Section FWiley

Published: Feb 1, 2013

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