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Expression, purification, crystallization and preliminary crystallographic analysis of chitinase A from Vibrio carchariae

Expression, purification, crystallization and preliminary crystallographic analysis of chitinase... Chitinase A of Vibrio carchariae was expressed in Escherichia coli M15 host cells as a 575‐amino‐acid fragment with full enzymatic activity using the pQE60 expression vector. The yield of the highly purified recombinant protein was approximately 70 mg per litre of bacterial culture. The molecular mass of the expressed protein was determined by HPLC/ESI–MS to be 63 770, including the hexahistidine tag. Crystals of recombinant chitinase A were grown to a suitable size for X‐ray structure analysis in a precipitant containing 10%(v/v) PEG 400, 0.1 M sodium acetate pH 4.6 and 0.125 M CaCl2. The crystals belonged to the tetragonal space group P422, with two molecules per asymmetric unit and unit‐cell parameters a = b = 127.64, c = 171.42 Å. A complete diffraction data set was collected to 2.14 Å resolution using a Rigaku/MSC R‐AXIS IV++ detector system mounted on an RU‐H3R rotating‐anode X‐ray generator. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Expression, purification, crystallization and preliminary crystallographic analysis of chitinase A from Vibrio carchariae

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References (2)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309105027831
pmid
16511189
Publisher site
See Article on Publisher Site

Abstract

Chitinase A of Vibrio carchariae was expressed in Escherichia coli M15 host cells as a 575‐amino‐acid fragment with full enzymatic activity using the pQE60 expression vector. The yield of the highly purified recombinant protein was approximately 70 mg per litre of bacterial culture. The molecular mass of the expressed protein was determined by HPLC/ESI–MS to be 63 770, including the hexahistidine tag. Crystals of recombinant chitinase A were grown to a suitable size for X‐ray structure analysis in a precipitant containing 10%(v/v) PEG 400, 0.1 M sodium acetate pH 4.6 and 0.125 M CaCl2. The crystals belonged to the tetragonal space group P422, with two molecules per asymmetric unit and unit‐cell parameters a = b = 127.64, c = 171.42 Å. A complete diffraction data set was collected to 2.14 Å resolution using a Rigaku/MSC R‐AXIS IV++ detector system mounted on an RU‐H3R rotating‐anode X‐ray generator.

Journal

Acta Crystallographica Section F – Wiley

Published: Oct 1, 2005

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