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Expression, purification and preliminary X‐ray crystallographic analysis of UDP‐galactopyranose mutase from Deinococcus radiodurans

Expression, purification and preliminary X‐ray crystallographic analysis of UDP‐galactopyranose... UDP‐galactopyranose mutase (UGM) catalyzes the interconversion of UDP‐galactopyranose and UDP‐galactofuranose. A UGM–substrate complex from Deinococccus radiodurans has been expressed, purified and crystallized. Crystals were obtained by the microbatch‐under‐oil method at room temperature. The crystals diffracted to 2.36 Å resolution at the Canadian Light Source The space group was found to be P212121, with unit‐cell parameters a = 134.0, b = 176.6, c = 221.6 Å. The initial structure solution was determined by molecular replacement using UGM from Mycobacterium tuberculosis (PDB code 1v0j) as a template model. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Expression, purification and preliminary X‐ray crystallographic analysis of UDP‐galactopyranose mutase from Deinococcus radiodurans

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References (13)

Publisher
Wiley
Copyright
International Union of Crystallography, 2009
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309109027754
pmid
19652355
Publisher site
See Article on Publisher Site

Abstract

UDP‐galactopyranose mutase (UGM) catalyzes the interconversion of UDP‐galactopyranose and UDP‐galactofuranose. A UGM–substrate complex from Deinococccus radiodurans has been expressed, purified and crystallized. Crystals were obtained by the microbatch‐under‐oil method at room temperature. The crystals diffracted to 2.36 Å resolution at the Canadian Light Source The space group was found to be P212121, with unit‐cell parameters a = 134.0, b = 176.6, c = 221.6 Å. The initial structure solution was determined by molecular replacement using UGM from Mycobacterium tuberculosis (PDB code 1v0j) as a template model.

Journal

Acta Crystallographica Section FWiley

Published: Aug 1, 2009

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