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- Publisher
- Wiley
- Copyright
- Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309106009274
- pmid
- 16582497
- Publisher site
- See Article on Publisher Site
Abstract
Macro domains or X domains are found as modules of multidomain proteins, but can also constitute a protein on their own. Recently, biochemical and structural studies of cellular macro domains have been performed, showing that they are active as ADP‐ribose‐1′′‐phosphatases. Macro domains are also present in a number of positive‐stranded RNA viruses, but their precise function in viral replication is still unknown. The major human pathogen severe acute respiratory syndrome coronavirus (SARS‐CoV) encodes 16 non‐structural proteins (nsps), one of which (nsp3) encompasses a macro domain. The SARS‐CoV nsp3 gene region corresponding to amino acids 182–355 has been cloned, expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P21, with unit‐cell parameters a = 37.5, b = 55.6, c = 108.9 Å, β = 91.4°, and the asymmetric unit contains either two or three molecules. Both native and selenomethionine‐labelled crystals diffract to 1.8 Å.
Journal
Acta Crystallographica Section F – Wiley
Published: Apr 1, 2006