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Exploration of early‐life candidate biomarkers for childhood asthma using antibody arrays

Exploration of early‐life candidate biomarkers for childhood asthma using antibody arrays asthma; plasma; biomarker; erythropoietin; Background: Proteomic approaches identifying biomarkers have been applied to soluble GP130 asthma to only a very limited extent. Methods: With an antibody array (RayBiotech, Norcross, GA, USA), the relative Correspondence intensity and rank differences of 444 proteins were compared in 24 plasma samples Serrine S. Lau, Department of obtained at age 3, 11 from children with and 12 without asthma diagnoses at ages 5 Pharmaceutical Sciences, Eugene and 9. Protein candidates identified by antibody array were quantitated by ELISA in Applebaum College of Pharmacy and Health an enlarged sample. Proteins found to differentiate children with and without asthma Sciences, Wayne State University, 259 Mack Ave., Detroit, MI 48201, USA were also examined for association with known Year 1 asthma risk factors, eczema, Tel.: +(313) 577-1574 and wheeze. Fax: +(313) 577-0457 Results: In the antibody array, four proteins had rank differences between asthma and E-mail: serrine.lau@wayne.edu non-asthma groups (FDR <0.1). By ELISA, mean log (s.e.m.) erythropoietin (EPO) level (IU/l) was lower (0.750  0.048 vs. 0.898  0.035; p = 0.006) and mean Present address (s.e.m.) soluble GP130 (sGP130) level (ng/ml) was higher in the asthma vs. the *Arizona State University, 4701 Thunderbird non-asthma group (302 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pediatric Allergy and Immunology Wiley

Exploration of early‐life candidate biomarkers for childhood asthma using antibody arrays

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References (33)

Publisher
Wiley
Copyright
Copyright © 2016 John Wiley & Sons A/S
ISSN
0905-6157
eISSN
1399-3038
DOI
10.1111/pai.12613
pmid
27434124
Publisher site
See Article on Publisher Site

Abstract

asthma; plasma; biomarker; erythropoietin; Background: Proteomic approaches identifying biomarkers have been applied to soluble GP130 asthma to only a very limited extent. Methods: With an antibody array (RayBiotech, Norcross, GA, USA), the relative Correspondence intensity and rank differences of 444 proteins were compared in 24 plasma samples Serrine S. Lau, Department of obtained at age 3, 11 from children with and 12 without asthma diagnoses at ages 5 Pharmaceutical Sciences, Eugene and 9. Protein candidates identified by antibody array were quantitated by ELISA in Applebaum College of Pharmacy and Health an enlarged sample. Proteins found to differentiate children with and without asthma Sciences, Wayne State University, 259 Mack Ave., Detroit, MI 48201, USA were also examined for association with known Year 1 asthma risk factors, eczema, Tel.: +(313) 577-1574 and wheeze. Fax: +(313) 577-0457 Results: In the antibody array, four proteins had rank differences between asthma and E-mail: serrine.lau@wayne.edu non-asthma groups (FDR <0.1). By ELISA, mean log (s.e.m.) erythropoietin (EPO) level (IU/l) was lower (0.750  0.048 vs. 0.898  0.035; p = 0.006) and mean Present address (s.e.m.) soluble GP130 (sGP130) level (ng/ml) was higher in the asthma vs. the *Arizona State University, 4701 Thunderbird non-asthma group (302

Journal

Pediatric Allergy and Immunology – Wiley

Published: Nov 1, 2016

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