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Evaluation of a water concentration method for the detection of infectious pancreatic necrosis virus in a fish hatchery

Evaluation of a water concentration method for the detection of infectious pancreatic necrosis... Summary A virus‐adsorption‐elution (viradel) method using electropositive microporous filters (Virosorb 1‐MDS) was used for the concentration and détéction of infectious pancreatic necrosis virus (IPNV) at a hatchery with a past history of IPNV infection. Samples of fish tissue and unconcentrated water were also examined for the presence of IPNV. Virus was isolated from three of the nine concentrated water samples taken from various locations at the hatchery. Virus was also isolated from pooled fish tissues, but not from unconcentrated water samples. Representative numbers of viruses isolated from water and fish tissues were examined for virus neutralization in the presence of anti‐IPNV amiserum; resistance to acid, ether, and heat; and replication in the presence of 5‐bromo‐2'‐deoxyuridine (BUDR). When tested by dot‐immunobinding assay using a panel of monoclonal antibodies (MABs), the viral isolates were found to belong to the West Buxton serotype of IPNV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Ichthyology Wiley

Evaluation of a water concentration method for the detection of infectious pancreatic necrosis virus in a fish hatchery

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References (12)

Publisher
Wiley
Copyright
Copyright © 1991 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0175-8659
eISSN
1439-0426
DOI
10.1111/j.1439-0426.1991.tb00517.x
Publisher site
See Article on Publisher Site

Abstract

Summary A virus‐adsorption‐elution (viradel) method using electropositive microporous filters (Virosorb 1‐MDS) was used for the concentration and détéction of infectious pancreatic necrosis virus (IPNV) at a hatchery with a past history of IPNV infection. Samples of fish tissue and unconcentrated water were also examined for the presence of IPNV. Virus was isolated from three of the nine concentrated water samples taken from various locations at the hatchery. Virus was also isolated from pooled fish tissues, but not from unconcentrated water samples. Representative numbers of viruses isolated from water and fish tissues were examined for virus neutralization in the presence of anti‐IPNV amiserum; resistance to acid, ether, and heat; and replication in the presence of 5‐bromo‐2'‐deoxyuridine (BUDR). When tested by dot‐immunobinding assay using a panel of monoclonal antibodies (MABs), the viral isolates were found to belong to the West Buxton serotype of IPNV.

Journal

Journal of Applied IchthyologyWiley

Published: Jun 1, 1991

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